A flow-cytometric method to investigate glutamate-receptor-sensitivity in whole blood platelets - results from healthy controls and patients with schizophrenia

Journal of Psychiatric Research
Paul C BaierDunja Hinze-Selch

Abstract

Hypofunction of glutamate receptors may contribute to the symptoms of schizophrenia. Human platelets express glutamate receptors and can serve as peripheral surrogate model for neuronal cells. Aim of this study was to establish a fast and sensitive flow-cytometric method to determine the glutamate-dependent kinetics of intracellular calcium ([Ca++]i) mobilization in platelets of schizophrenic patients. Glutamate stimulated [Ca++]i response was measured with a flow-cytometer in anti-CD-41a-labelled whole blood platelets of treated schizophrenic patients (n=18) and controls (n=18). In two control experiments the NMDA-receptor antagonist MK-801 and the dopamine antagonist amisulpride, respectively, were added to probes from healthy subjects. Stimulation with glutamate led dose-dependently to a mobilization of [Ca++]i in both healthy controls and patients. This effect was significantly reduced in patients. In vitro NMDA-antagonism inhibited the glutamate response, whereas dopamine-antagonism had no effect. Our flow-cytometric method allows to measure glutamate-receptor mediated [Ca++]i response in whole blood platelets, without requiring platelet rich preparations. The reduced glutamate-response in the patients was not explained by...Continue Reading

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Citations

Nov 30, 2013·Biological Psychiatry·Akiko Hayashi-TakagiKazuya Iwamoto
Oct 23, 2010·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Marisa MöllerBrian H Harvey
Sep 4, 2010·Progress in Neuro-psychopharmacology & Biological Psychiatry·Lukasz BojarskiUrszula Wojda
Mar 7, 2009·Journal of Psychiatric Research·Maurice M Ohayon
Feb 15, 2014·The International Journal of Neuropsychopharmacology·Hendrik WesselingSabine Bahn
Apr 9, 2016·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·David PellerinFrançois Corbin
Nov 1, 2013·World Journal of Psychiatry·Eyal Asor, Dorit Ben-Shachar
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Aug 5, 2010·Journal of Agricultural and Food Chemistry·Yuan-Shu LaiTz-Chong Chou
May 18, 2021·Biochemistry. Biokhimii︠a︡·Irina S BokshaGulnur Sh Burbaeva

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