A Fluidic Culture Platform for Spatially Patterned Cell Growth, Differentiation, and Cocultures

Tissue Engineering. Part a
Josephine LembongJohn P Fisher

Abstract

Stem cell cultures within perfusion bioreactors, while efficient in obtaining cell numbers, often lack the similarity to native tissues and consequently cell phenotype. We develop a three-dimensional (3D)-printed fluidic chamber for dynamic stem cell culture, with emphasis on control over flow and substrate curvature in a 3D environment, two physiologic features of native tissues. The chamber geometry, consisting of an array of vertical cylindrical pillars, facilitates actin-mediated localization of human mesenchymal stem cells (hMSCs) within ∼200 μm distance from the pillars, enabling spatial patterning of hMSCs and endothelial cells in cocultures and subsequent modulation of calcium signaling between these two essential cell types in the bone marrow microenvironment. Flow-enhanced osteogenic differentiation of hMSCs in growth media imposes spatial variations of alkaline phosphatase expression, which positively correlates with local shear stress. Proliferation of hMSCs is maintained within the chamber, exceeding the cell expansion in conventional static culture. The capability to manipulate cell spatial patterning, differentiation, and 3D tissue formation through geometry and flow demonstrates the culture chamber's relevant ch...Continue Reading

References

Jul 29, 1998·Medical Engineering & Physics·J Y RhoP Zioupos
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Sep 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gregory N BancroftAntonios G Mikos
May 3, 2003·Yonsei Medical Journal·Hyongbum KimHwal Suh
Oct 24, 2003·Nature·L M CalviD T Scadden
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vassilios I SikavitsasAntonios G Mikos
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xiaojun YuCato T Laurencin
Oct 12, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ying Jun LiChristopher R Jacobs
Jan 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Darnell KaiglerDavid J Mooney
Feb 16, 2005·Annals of Biomedical Engineering·Vassilios I SikavitsasAntonios G Mikos
Jul 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Celeste M NelsonChristopher S Chen
Nov 4, 2005·American Journal of Physiology. Cell Physiology·Ryan C RiddleHenry J Donahue
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Néha DattaAntonios G Mikos
May 4, 2006·The Journal of Clinical Investigation·Tong Yin, Linheng Li
Jul 1, 2006·Nature·David T Scadden
Jul 28, 2006·Nature·Jamil El-AliKlavs F Jensen
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kyle KurpinskiSong Li
Jan 12, 2007·Annals of Biomedical Engineering·Jose F Alvarez-BarretoVassilios I Sikavitsas
Apr 26, 2007·Macromolecular Bioscience·Jose F Alvarez-Barreto, Vassilios I Sikavitsas
Sep 25, 2007·Nature Materials·Matthew J DalbyRichard O C Oreffo
Oct 31, 2007·Journal of Biomedical Materials Research. Part a·S ScaglioneI Martin
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·D Leanne Jones, Amy J Wagers
Mar 20, 2008·Journal of the Royal Society, Interface·Monika RumplerPeter Fratzl
Jul 16, 2008·Tissue Engineering. Part a·Warren L GraysonGordana Vunjak-Novakovic
Aug 16, 2008·Stem Cells·Sami Alom Ruiz, Christopher S Chen
Oct 11, 2008·Journal of Cellular Physiology·Tae-Jin KimYingxiao Wang
Dec 18, 2008·The Journal of Pathology·M R WalkerT S Stappenbeck
Mar 14, 2009·Journal of Tissue Engineering and Regenerative Medicine·Maritie GrellierJoëlle Amédée
May 7, 2009·Acta Pharmacologica Sinica·Jian-de DongJian Zhang
Oct 9, 2009·Tissue Engineering. Part C, Methods·Jessica E FrithPaul G Genever
Oct 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Warren L GraysonGordana Vunjak-Novakovic
Dec 29, 2009·Cellular and Molecular Bioengineering·Kyle KurpinskiSong Li
Jan 9, 2010·Journal of Tissue Engineering and Regenerative Medicine·Luuk M JanssenJosé A U Hardillo
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kristopher A KilianMilan Mrksich
Jun 12, 2010·Stem Cells and Development·Fatima A SalehPaul G Genever
Jul 20, 2010·Journal of Dental Research·I S KimS J Hwang
Sep 28, 2010·Regenerative Medicine·Gregory YourekGwendolen C Reilly
Sep 30, 2010·Journal of Biomedical Materials Research. Part a·Brad M Isaacson, Roy D Bloebaum
Oct 12, 2010·Tissue Engineering. Part C, Methods·Andrew B Yeatts, John P Fisher

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Citations

Oct 29, 2018·Biotechnology Letters·Shehnaz AhmedJonathan W Aylott
Mar 2, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Max J LermanJohn P Fisher
Jan 24, 2020·Tissue Engineering. Part C, Methods·Max J LermanJohn P Fisher
Sep 11, 2019·Biotechnology and Bioengineering·Javier NavarroJohn P Fisher
Dec 1, 2018·Applied Physics Reviews·Max J LermanJohn P Fisher
Nov 6, 2020·Biomaterials Science·Kamol DeyLuciana Sartore
Feb 6, 2021·Trends in Biotechnology·Nathalie Picollet-D'hahanSéverine Le Gac
Nov 18, 2021·Advanced Science·Claire BomkampElizabeth A Specht
Feb 2, 2022·Archives of Toxicology·Katharina S NitscheHans Bouwmeester

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Methods Mentioned

BETA
PCR

Software Mentioned

ImageJ
MATLAB
EnvisionTEC Perfactory
SolidWorks

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