A fluorescence in situ hybridization (FISH) microfluidic platform for detection of HER2 amplification in cancer cells

Biosensors & Bioelectronics
Kai-Jie KaoGwo-Bin Lee

Abstract

Over-expression/amplification of human epidermal growth factor receptors 2 (HER2) is a verified therapeutic biomarker for breast and gastric cancers. HER2 is also served as prognostic biomarker for gastric cancer because HER2 over-expression is associated with a 5-10% increase in cancer related death of gastric cancer. Cancer patients exhibiting HER2 over-expression can significantly improve their overall survival rates by taking the targeting drug Herceptin, which directly targets HER2. However, Herceptin has limited functions toward patients without HER2 over-expression and therefore it needs a highly specific and accurate detection method for diagnosis of HER2 over-expression. Currently, fluorescence in situ hybridization (FISH) technique is routinely employed to detect HER2 amplification. However, it is a labor-intensive, time-consuming hybridization process and is relatively costly. Furthermore, well-trained personnel are required to operate the delicate and complicate process. More importantly, it may take 1-2 days for well-trained personnel to perform a whole FISH assay. Given these limitations, we developed a new, integrated microfluidic FISH system capable of automating the entire FISH protocol which could be performed...Continue Reading

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Citations

Sep 23, 2016·Frontiers in Cell and Developmental Biology·Chenghua CuiPeining Li
Nov 29, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Huu Tuan NguyenMartin Am Gijs
Jun 12, 2018·Biomicrofluidics·D Huber, G V Kaigala
Mar 19, 2019·Annual Review of Biomedical Engineering·Yanxiang DengRong Fan
Dec 14, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Vivek Priy DaveDang Duong Bang
Oct 3, 2018·Biomicrofluidics·Dong Jun LeeTony Jun Huang
Jan 23, 2020·Medical Microbiology and Immunology·Pablo Rodriguez-MateosNicole Pamme

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