A fluorescence resonance energy transfer (FRET) based "Turn-On" nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to α-glucosidase inhibitor screening

Biosensors & Bioelectronics
Guoliang LiYongning Wu

Abstract

The medicines targeted at α-glucosidase played an important role in anti-diabetes and anti-HIV therapy. Unfortunately, the method based on fluorescent assay strategy for α-glucosidase inhibitor screening remains poorly investigated. In this study, a novel "Turn On" fluorescence sensor platform has been developed for trace α-glucosidase inhibitor screening from natural medicines. Firstly, carbon dots were prepared by one-pot synthesis and used as the signal output. Combining with the carbon dots, cobalt oxyhydroxide (CoOOH) nanoflakes were employed to build the fluorescence resonance energy transfer (FRET) based sensor platform. Secondly, L-ascorbic acid-2-O-α-D-glucopyranosyl (AAG) was innovatively introduced as α-glucosidase substrate. With hydrolysis of AAG by α-glucosidase, ascorbic acids (AA) were released that can rapidly reduce CoOOH nanoflakes to Co(2+), and then FRET was stopped accompanying with the fluorescence recovery of CDs. The sensor platform was ultrasensitive to AA with a detection limit of 5 nM, ensuring the sensitive monitoring of enzyme activity. Acarbose was used as the inhibitor model and its inhibition rate is proportional to the logarithm of concentration in range of 10(-9)-10(-3)M with the correlation c...Continue Reading

References

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Citations

Oct 19, 2017·Luminescence : the Journal of Biological and Chemical Luminescence·Junjian LiWei Dong
Sep 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Taili ShaoKaoshan Chen
May 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shi Gang LiuHong Qun Luo
Nov 21, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaohua ZhuShouzhuo Yao
Nov 12, 2019·Frontiers in Cellular Neuroscience·Anna V LeopoldVladislav V Verkhusha
May 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yang SongYuehe Lin
Jun 11, 2021·Mikrochimica Acta·Jiabao ZhangXingguang Su
Jun 17, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Xucan YuanZhili Xiong
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tao TianFeng-Qing Yang
Aug 20, 2019·ACS Applied Materials & Interfaces·Yingying ZhongSuqing Zhao
Nov 7, 2021·Luminescence : the Journal of Biological and Chemical Luminescence·Neda AziziNaser Samadi

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