A Fluorescent Carbon Nanotube Sensor Detects the Metastatic Prostate Cancer Biomarker uPA

ACS Sensors
Ryan M WilliamsDaniel A Heller

Abstract

Therapeutic outcomes in patients with prostate cancer are hindered by the inability to discern indolent versus aggressive disease. To address this problem, we developed a quantitative fluorescent nanosensor for the cancer biomarker urokinase plasminogen activator (uPA). We used the unique fluorescent characteristics of single-walled carbon nanotubes (SWCNT) to engineer an optical sensor that responds to uPA via optical bandgap modulation in complex protein environments. The sensing characteristics of this construct were modulated by passivation of the hydrophobic SWCNT surface with bovine serum albumin (BSA). The sensor enabled quantitative detection of known uPA concentrations in human blood products. These experiments potentiate future use of this technology as a rapid, point-of-care sensor for biomarker measurements in patient fluid samples. We expect that further work will develop a method to discern aggressive vs indolent prostate cancer and reduce overtreatment of this disease.

References

Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J D RodwellT J McKearn
Apr 15, 2003·Nature Materials·Ming ZhengNancy G Tassi
Apr 20, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Beata Sweryda-KrawiecJames J Hickman
Sep 20, 2005·Nature Biotechnology·Gengfeng ZhengCharles M Lieber
Aug 17, 2006·Journal of the National Cancer Institute·Ian M ThompsonCharles A Coltman
Aug 24, 2006·Journal of the American Chemical Society·Xin YuJames F Rusling
Dec 13, 2006·The Journal of Urology·E W SteyerbergF H Schröder
Feb 1, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Shahrokh F ShariatKevin M Slawin
Oct 24, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Lyndsay HarrisUNKNOWN American Society of Clinical Oncology
Jul 22, 2008·Nature Biotechnology·Jagath R JunutulaWilliam Mallet
Feb 7, 2009·Nature Nanotechnology·Daniel A HellerMichael S Strano
Mar 12, 2009·Journal of the National Cancer Institute·Gerrit DraismaHarry de Koning
Oct 1, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Y L JeyachandranJ A Mielczarski
Dec 29, 2009·Frontiers in Bioscience (Scholar Edition)·Ryan M Williams, Rajesh K Naz
Feb 5, 2010·Biosensors & Bioelectronics·Guoqing WangJaebum Choo
Mar 3, 2010·Biochimica Et Biophysica Acta·Shun-rong JiXian-jun Yu
May 11, 2011·Proceedings of the National Academy of Sciences of the United States of America·Daniel A HellerMichael S Strano
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kevin WelsherHongjie Dai
Mar 31, 2012·Science Translational Medicine·John R PrensnerArul M Chinnaiyan
Sep 12, 2012·Annals of Internal Medicine·Virginia A Moyer, UNKNOWN U.S. Preventive Services Task Force
Nov 20, 2012·Nature Medicine·Guosong HongHongjie Dai
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sutapa BaruaSamir Mitragotri
Mar 19, 2013·Annual Review of Biochemistry·Serge Muyldermans
Apr 24, 2013·Current Opinion in Endocrinology, Diabetes, and Obesity·Laurence Klotz
Apr 25, 2013·Journal of the American Chemical Society·Constantine Y KhripinMing Zheng
Nov 5, 2013·Nature Nanotechnology·Nicole M IversonMichael S Strano
Jan 21, 2014·European Urology·Stacy LoebRuth Etzioni
May 9, 2014·The Lancet Oncology·Laura J EssermanSudhir Srivastava

❮ Previous
Next ❯

Citations

Dec 11, 2019·Sensors·Adi Hendler-Neumark, Gili Bisker
Oct 17, 2019·Frontiers in Chemistry·Alice J Gillen, Ardemis A Boghossian
Jun 1, 2019·Nature Reviews. Chemistry·Alexandra H BrozenaYuHuang Wang
Jan 9, 2021·Nature Chemical Biology·Ryan M WilliamsDaniel A Heller
Jun 11, 2021·Frontiers in Chemistry·Gurpal SinghRavi Pratap Barnwal
Jul 3, 2021·Biomolecules·Mark FreeleyMatteo Palma
Jul 21, 2021·Chemical Reviews·W Russ AlgarHyungki Kim
Oct 30, 2020·Nano Letters·Zvi YaariDaniel A Heller
Sep 22, 2020·Nano Letters·Ryan M WilliamsDaniel A Heller

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.