A Fluorescent Cell-Based System for Imaging Zika Virus Infection in Real-Time.

Viruses
Michael J McFaddenStacy M Horner

Abstract

Zika virus (ZIKV) is a re-emerging flavivirus that is transmitted to humans through the bite of an infected mosquito or through sexual contact with an infected partner. ZIKV infection during pregnancy has been associated with numerous fetal abnormalities, including prenatal lethality and microcephaly. However, until recent outbreaks in the Americas, ZIKV has been relatively understudied, and therefore the biology and pathogenesis of ZIKV infection remain incompletely understood. Better methods to study ZIKV infection in live cells could enhance our understanding of the biology of ZIKV and the mechanisms by which ZIKV contributes to fetal abnormalities. To this end, we developed a fluorescent cell-based reporter system allowing for live imaging of ZIKV-infected cells. This system utilizes the protease activity of the ZIKV non-structural proteins 2B and 3 (NS2B-NS3) to specifically mark virus-infected cells. Here, we demonstrate the utility of this fluorescent reporter for identifying cells infected by ZIKV strains of two lineages. Further, we use this system to determine that apoptosis is induced in cells directly infected with ZIKV in a cell-autonomous manner. Ultimately, approaches that can directly track ZIKV-infected cells a...Continue Reading

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Citations

Jul 10, 2019·Annual Review of Neuroscience·Kimberly M ChristianGuo-Li Ming
Oct 31, 2020·International Journal of Molecular Sciences·Alexander A DolskiyDmitry V Yudkin
Apr 13, 2021·Frontiers in Microbiology·Yuhong PanRenyong Jia

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Datasets Mentioned

BETA
KU501215
KX087101.3
KU955591
KU725663.1

Methods Mentioned

BETA
fluorescence microscopy
nuclear translocation
PCR
transfections
transfection
X-ray
confocal microscopy
fluorescence-activated cell sorting

Software Mentioned

NIH Fiji / ImageJ
NIH
Zeiss ZEN
Zen
ImageJ
Fiji
Zen Blue

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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