A fluorescent imaging assay of cast in renal disease based on graphene quantum dots and Fe3O4 nanoparticles

Clinica Chimica Acta; International Journal of Clinical Chemistry
Dongneng JiangXiaoyun Pu

Abstract

Renal disease has become a global public health problem. Cast is a useful disease marker of kidney injury and renal failure. Hence, a cast-targeted fluorescent imaging assay is developed for the laboratory diagnosis of renal disease. Firstly, graphene quantum dots (GQDs) were stripped from graphene oxide sheets and amine-modified. Then, anti-human IgG antibody was conjugated with Fe3O4 nanoparticles to identify the cast in urine. Furthermore, the modified GQDs were linked onto the surface of Fe3O4/anti-IgG nanocomposites. Lastly, this Fe3O4/GQD fluorescent probe was added into the sample to detect the cast through fluorescent imaging. Preliminary application of this probe in clinical detection showed that the common types of casts in urine (including RBC, WBC, fatty and granular casts) could be detected by this fluorescent imaging assay. The method has the advantages of fast speed, high sensitivity (lowest detection limit to 2 casts/ml), good selectivity, and wide linear range (2-2000 casts/ml). Regression analysis also showed that there was a good linear relationship (y=0.9495×+10.974, R(2)=0.9879) between the fluorescent counts and the casts in urine. This cast-targeted fluorescent imaging assay may be a potential method for ...Continue Reading

References

Jan 1, 1993·American Journal of Obstetrics and Gynecology·E D GalleryA Z Györy
Nov 1, 1996·Archives of Pediatrics & Adolescent Medicine·M RasoulpourG S Burke
Mar 1, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Matthias P HörlMartin Tepel
Nov 26, 2008·Chemical Communications : Chem Comm·Murat SunbulJun Yin
Jan 26, 2013·ACS Nano·Kenrick J WilliamsXiaoyang Zhu
Apr 11, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ying-Yong Zhao
Apr 29, 2014·The Analyst·T R PisanicT H Wang
Sep 3, 2014·Advanced Materials·Lihong JingMingyuan Gao
Sep 6, 2014·Nanoscale·Lan FengLan-Sun Zheng
Oct 15, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hun KimChulmin Joo
Nov 9, 2014·ACS Nano·Saumya SaurabhMarcel P Bruchez
Dec 3, 2014·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Mazdak A KhalighiShane M Meehan

❮ Previous
Next ❯

Citations

Feb 23, 2020·Sensors·Bhargav D Mansuriya, Zeynep Altintas
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Amirhossein AlaghmandfardAlexander Seifalian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.