A fluorescent immunochromatographic strip test using Quantum Dots for fumonisins detection

Talanta
F Di NardoClaudio Baggiani

Abstract

A fluorescent immunochromatographic strip test (ICST) based on the use of Quantum Dots (QD) was developed and applied to detect fumonisins in maize samples. A limit of detection for fumonisin B1 of 2.8 µg L(-1) was achieved, with an analytical working range of 3-350 µg L(-1), corresponding to 30-3500 µg kg(-1) in maize flour samples, according with the extraction procedure. The time required to perform the analysis was 22 min, including sample preparation. Recovery values in the range from 91.4% to 105.4% with coefficients of variation not exceeding 5% were obtained for fortified and naturally contaminated maize flour samples. To evaluate the possible improvements due to the use of QD for ICST technology, we performed a direct comparison of the proposed QD-ICST to a gold nanoparticles- and a chemiluminescent-ICST previously developed for fumonisins detection, in which the same immunoreagents were employed.

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Citations

Dec 13, 2016·Journal of Agricultural and Food Chemistry·Astrid FoubertSarah De Saeger
Jan 11, 2017·Critical Reviews in Food Science and Nutrition·Pranav TripathiSeema Nara
Jul 10, 2018·Mikrochimica Acta·Jing ZhangYulin Deng
Jan 15, 2020·Journal of Healthcare Engineering·Lei ShaoPengyuan Zhang
Dec 11, 2019·Talanta·Ruchita ShandilyaPradyumna Kumar Mishra
Jan 5, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Poorvisha Ravi, Muthupandian Ganesan
Oct 28, 2017·Journal of Agricultural and Food Chemistry·Shuangjie WangGuonian Zhu

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