A fluorescent membrane tension probe

Nature Chemistry
Adai ColomAurélien Roux

Abstract

Cells and organelles are delimited by lipid bilayers in which high deformability is essential to many cell processes, including motility, endocytosis and cell division. Membrane tension is therefore a major regulator of the cell processes that remodel membranes, albeit one that is very hard to measure in vivo. Here we show that a planarizable push-pull fluorescent probe called FliptR (fluorescent lipid tension reporter) can monitor changes in membrane tension by changing its fluorescence lifetime as a function of the twist between its fluorescent groups. The fluorescence lifetime depends linearly on membrane tension within cells, enabling an easy quantification of membrane tension by fluorescence lifetime imaging microscopy. We further show, using model membranes, that this linear dependency between lifetime of the probe and membrane tension relies on a membrane-tension-dependent lipid phase separation. We also provide calibration curves that enable accurate measurement of membrane tension using fluorescence lifetime imaging microscopy.

References

Jan 1, 1988·Journal of Cellular Biochemistry·G van Meer, K Simons
Nov 1, 1973·Zeitschrift Für Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie·W Helfrich
Jul 17, 1998·Biochimica Et Biophysica Acta·R Koynova, M Caffrey
Jun 27, 2000·Biophysical Journal·W RawiczE Evans
Sep 26, 2003·Biophysical Journal·Evan EvansWieslawa Rawicz
Jul 22, 2006·Biochemical and Biophysical Research Communications·Yan-Liang ZhangMirianas Chachisvilis
Jun 4, 2008·Physical Review Letters·Miho YanagisawaTakashi Taniguchi
May 22, 2009·Molecular Biology of the Cell·Nils C GauthierMichael P Sheetz
Jan 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Julio L SampaioAndrej Shevchenko
Apr 1, 2009·Nature Chemistry·Marina K KuimovaPeter R Ogilby
Jul 5, 2011·Biochimica Et Biophysica Acta·Dora Toledo WarshaviakMirianas Chachisvilis
Aug 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Nils C GauthierMichael P Sheetz
Aug 16, 2011·Nature Cell Biology·Steeve BoulantTomas Kirchhausen
Apr 14, 2012·Organic & Biomolecular Chemistry·David Alonso DovalStefan Matile
May 16, 2012·Frontiers in Physiology·Kamila OglęckaRachel S Kraut
Aug 28, 2012·Trends in Cell Biology·Nils C GauthierMichael P Sheetz
Jan 23, 2013·Biochimica Et Biophysica Acta·Robin S PetruzieloGerald W Feigenson
Jul 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Thomas A MastersNils C Gauthier
Jul 9, 2013·Current Biology : CB·Arnon D LieberKinneret Keren
May 6, 2014·Current Biology : CB·Chiara GabellaAlexander B Verkhovsky
Jan 15, 2015·Journal of the American Chemical Society·Marta Dal MolinStefan Matile
Feb 24, 2015·Physical Chemistry Chemical Physics : PCCP·Aurimas VyšniauskasMarina K Kuimova
Apr 12, 2015·Biophysical Journal·Arnon D LieberKinneret Keren
Feb 13, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·James C S HoAtul N Parikh
Apr 30, 2016·Journal of Materials Chemistry. C, Materials for Optical and Electronic Devices·Mark A Haidekker, Emmanuel A Theodorakis
Nov 17, 2016·Biophysical Journal·Sayed Ul Alam ShiblyMasahito Yamazaki
Dec 3, 2016·Chemical Communications : Chem Comm·Saeideh SoleimanpourStefan Matile
May 10, 2017·Chemistry : a European Journal·Aurimas VyšniauskasMarina K Kuimova
Jun 6, 2017·Chemical Science·Peter S SherinMarina K Kuimova
Jul 8, 2017·Beilstein Journal of Organic Chemistry·Frederik NeuhausAndreas Zumbuehl
Jul 9, 2017·The Journal of Cell Biology·Bruno PontesNils C Gauthier
Jul 12, 2017·Chemical Society Reviews·Dongdong SuYoung-Tae Chang
Jul 27, 2017·Biochemistry·Rishikesh U Kulkarni, Evan W Miller

❮ Previous
Next ❯

Citations

Apr 13, 2019·Physical Biology·Duco BlankenChristophe Danelon
Jun 28, 2019·Chemical Communications : Chem Comm·Jasmine Bernal-EscalanteArturo Jiménez-Sánchez
Aug 21, 2019·Micromachines·Qian FengBenoît Kornmann
Aug 9, 2019·Angewandte Chemie·Dmytro I DanylchukAndrey S Klymchenko
Oct 23, 2019·Chemistry : a European Journal·Karolina StrakovaStefan Matile
Dec 12, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Adam E Cohen, Zheng Shi
Jan 11, 2020·ChemistryOpen·Xiang ZhangStefan Matile
Feb 23, 2020·PLoS Pathogens·Charles A CoomerSergi Padilla-Parra
Sep 21, 2019·Angewandte Chemie·Mariano MacchioneStefan Matile
Jun 13, 2020·Chemical Communications : Chem Comm·Keiichi ImatoNaoya Takeda
Jun 20, 2020·Chemistry : a European Journal·Tiddo Jonathan MooibroekJarl Ivar van der Vlugt
Jun 27, 2020·Chemical Communications : Chem Comm·Ling FengHongyan Sun
May 28, 2019·The Journal of Cell Biology·Margot RiggiAurélien Roux
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Jian ZhangCynthia A Reinhart-King
Apr 5, 2020·Viruses·Pablo CarravillaChristian Eggeling
May 13, 2020·Journal of Cell Science·Margot RiggiRobbie Loewith
May 10, 2020·Nature Communications·ShengPeng WangStefan Offermanns
Sep 29, 2020·Frontiers in Cell and Developmental Biology·Takunori Minegishi, Naoyuki Inagaki
Aug 31, 2020·Chemical Science·Giuseppe LicariEmad Tajkhorshid
May 16, 2019·Biochemical Society Transactions·Sarah J RoutledgeAlan D Goddard
Jan 15, 2020·Advanced Biosystems·Qi ShenChenxiang Lin
Aug 6, 2020·Nature Cell Biology·Vincent MercierAurélien Roux
May 30, 2019·Frontiers in Chemistry·Zuhai LeiYoujun Yang
Aug 23, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Anabel-Lise Le RouxPere Roca-Cusachs
Jan 22, 2020·Nature Reviews. Microbiology·Yves F Dufrêne, Alexandre Persat
Mar 10, 2020·F1000Research·Ann Sutherland, Alyssa Lesko
Jul 17, 2020·Proceedings of the National Academy of Sciences of the United States of America·Lucile MichelsJoris Sprakel
Aug 6, 2020·Nature Cell Biology·Robert C Piper
Nov 26, 2020·Journal of Liposome Research·Chandra Has
Nov 27, 2020·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Tiddo Jonathan Mooibroek
Nov 18, 2019·Biochimica Et Biophysica Acta. General Subjects·Melissa C PiontekWouter H Roos
Oct 15, 2019·Developmental Cell·Joseph H R HetmanskiPatrick T Caswell
Dec 10, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Lucie OberhauserPierre Maechler
Feb 4, 2021·Journal of the Royal Society, Interface·Y HuangG G Fuller
Jan 20, 2020·Biophysical Journal·Amirali Hossein, Markus Deserno
Jan 26, 2021·Frontiers in Cell and Developmental Biology·Amra Saric, Spencer A Freeman
Jan 26, 2021·Frontiers in Bioengineering and Biotechnology·Fabrizio A PennacchioPaolo Maiuri

❮ Previous
Next ❯

Methods Mentioned

BETA
optical tweezers
PMA

Software Mentioned

SymPhoTime
FliptR
ImageJ
SymPho Time64
PicoQuant

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.