PMID: 15387378Sep 25, 2004Paper

A fluorescent orthotopic model of metastatic cervical carcinoma

Clinical & Experimental Metastasis
Rob A Cairns, Richard Hill

Abstract

Currently, there is no mouse model of cervical cancer that allows for the study of the later stages of the disease, including metastasis. We report here the development of an orthotopic model of human cervical carcinoma in which tumor fragments are surgically implanted into the cervix of SCID mice. The human cervical carcinoma cell lines used in this study (CaSki, ME-180, and SiHa) have been engineered to stably express the fluorescent proteins enhanced green fluorescent protein (EGFP) or DsRed2, allowing for in vivo optical monitoring of tumor growth and metastasis. The cervical implants develop into large intraperitoneal masses involving the entire reproductive tract, with little local invasion of other abdominal structures. These tumors metastasize initially to local lymph nodes and later to lung, a pattern consistent with the clinical course of the disease. It was found that the use of the DsRed2 protein as a fluorescent marker has distinct advantages over EGFP due to the wavelength of its emission spectrum (575-625 nm vs 500-550 nm). Tissue penetration of light at this wavelength is greater, and the auto-fluorescence of mouse tissues is less intense, resulting in an enhanced signal to noise ratio compared to results obtain...Continue Reading

Citations

Oct 2, 2007·Apoptosis : an International Journal on Programmed Cell Death·Takeshi HagaJoel M Palefsky
Jan 12, 2008·Microvascular Research·Michael MilosevicRichard P Hill
May 19, 2009·Journal of Neuroscience Methods·Xuepeng ZhangMichael Chopp
Apr 12, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sandra N EkdawiChristine Allen
Feb 2, 2013·Acta Cirúrgica Brasileira·Heriton Marcelo Ribeiro AntonioDaniel Guimarães Tiezzi
Dec 20, 2017·Frontiers in Pharmacology·Valéry L PayenPierre Sonveaux
Mar 25, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Naz Chaudary, Richard P Hill
Sep 26, 2017·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Wen-Fei WeiWei Wang
Jun 28, 2014·International Journal of Oncology·Dan LiuQing-Lei Gao
Sep 21, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hilda MujcicBradly G Wouters
Jun 8, 2011·Cancer Research·Jeevisha BajajSudhir Krishna
Apr 16, 2021·Advanced Drug Delivery Reviews·Xue WangZhigang Xie
Aug 16, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Martin EiblmaierCarolyn J Anderson

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