A Fluorescently Labeled Marburg Virus Glycoprotein as a New Tool to Study Viral Transport and Assembly

The Journal of Infectious Diseases
Eva MittlerStephan Becker

Abstract

The single surface glycoprotein (GP) of filoviruses is indispensable for recognition of its cellular receptor and infection of target cells. To study the intracellular trafficking of GP by using live-cell imaging, the mucin-like domain of Marburg virus (MARV) GP was replaced by the fluorophore mCherry (GP∆MLD_mCherry). Intracellular distribution, surface transport, and recruitment of GP∆MLD_mCherry into virus-like particles were similar to observations for wild-type GP. Using reverse genetics, we generated a recombinant MARV expressing GP∆MLD_mCherry (recMARV MARVGP∆MLD_mCherry). Time-lapse microscopy of recMARV MARVGP∆MLD_mCherry-infected cells revealed that GP∆MLD_mCherry-positive vesicles were transported to the cell surface in a tubulin-dependent manner. Moreover, dual-color live-cell imaging revealed cotransport of GPΔMLD_mCherry and VP40 and their colocalization at the plasma membrane. In this proof-of-concept study we showed that the newly developed GP∆MLD_mCherry is a promising tool to elucidate intracellular trafficking and assembly pathways of MARV.

References

Aug 1, 1992·Glycobiology·H GeyerR Geyer
Sep 1, 1996·The Journal of Hand Surgery·K HirakawaA H Wilde
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·V E VolchkovH D Klenk
Feb 23, 2000·Virology·V E VolchkovH Feldmann
Apr 1, 1995·Virology·C FunkeE Mühlberger
Jan 28, 2003·BioTechniques·Nancy C HawkinsChristopher T Beh
Oct 28, 2004·Journal of Virology·Larissa KolesnikovaStephan Becker
Jan 15, 2005·Journal of Virology·Thomas HoenenWinfried Weissenhorn
Apr 2, 2005·Nature Cell Biology·Thomas ForestJoel D Baines
Apr 16, 2005·Science·Kartik ChandranJames M Cunningham
Oct 18, 2005·Journal of Virology·Sandra BambergStephan Becker
Apr 6, 2006·The Journal of Biological Chemistry·Jens H KuhnMichael Farzan
Sep 23, 2006·Virology·Balaji ManicassamyLijun Rong
Dec 5, 2006·Cellular Microbiology·Larissa KolesnikovaStephan Becker
Dec 6, 2007·The Journal of Infectious Diseases·Larissa KolesnikovaStephan Becker
Jan 15, 2010·The Journal of General Virology·Jörg WenigenrathStephan Becker
May 6, 2010·PLoS Pathogens·Sonja WelschJohn A G Briggs
Aug 6, 2010·Journal of Virology·Sheli R RadoshitzkySina Bavari
Jun 18, 2011·Journal of Virology·Eva MittlerStephan Becker
Aug 26, 2011·Nature·Jan E CaretteThijn R Brummelkamp
Aug 24, 2012·Journal of Virology·Thomas HoenenHeinz Feldmann
Nov 29, 2012·Cellular Microbiology·Eva MittlerStephan Becker
Feb 6, 2013·Scientific Reports·Asuka NanboYoshihiro Kawaoka
Aug 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Gordian SchudtStephan Becker
Feb 11, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·V RougeronE M Leroy
Feb 28, 2015·Cell·Takao HashiguchiErica Ollmann Saphire
Jan 18, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yuki TakamatsuStephan Becker

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Citations

Oct 9, 2019·Nature Medicine·Stefanie A EhrhardtFlorian Klein
May 28, 2019·F1000Research·Judith OlejnikAdam J Hume
Feb 16, 2020·Cell Host & Microbe·Hadas Cohen-DvashiRon Diskin
Mar 14, 2021·Bulletin of Experimental Biology and Medicine·N V VolkovaD N Shcherbakov

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