A fluorimetric assay for angiotensin-I converting enzyme in human serum

Clinica Chimica Acta; International Journal of Clinical Chemistry
A CarmelA Yaron

Abstract

A fluorimetric method is described for a simple, sensitive and reproducible assay for angiotensin-I converting enzyme in human and guinea pig sera. The very weak fluorescent substrate o-aminobenzoylglycyl-p-nitro-L-phenylalanyl-L-proline is enzymatically hydrolyzed, producing the highly fluorescent o-aminobenzoylglycine that is quantitatively determined by spectrofluorimetry. Dependence of activity on substrate concentration, amount of serum, time of incubation and pH were investigated. The KM value for the substrate is 0.1 and 0.032 mM for the human and guinea pig serum enzyme, respectively. The mean value of serum angiotensin-I converting enzyme for 16 normal adult persons was 2.56 +/- 0.10 (S.E.) with a standard deviation of 0.81 nmol/min/ml serum.

References

Aug 31, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·H M NeelsM E van Sande
Mar 23, 1992·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Y EzraD Navot
Dec 30, 1996·Journal of Biochemical and Biophysical Methods·A A Gershkovich, V V Kholodovych
Aug 1, 1983·Journal of Clinical Pathology·P R StuddyR Bird
Dec 1, 1983·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·F Boomsma, M A Schalekamp
Feb 14, 2006·American Journal of Respiratory Cell and Molecular Biology·Julio C ValenciaJoel Moss
Jul 1, 1984·Analytical Biochemistry·M S KapiloffS H Snyder
Jan 1, 1985·Clinical and Experimental Hypertension. Part A, Theory and Practice·A KnechtT Rosenthal
Sep 1, 1984·Scandinavian Journal of Clinical and Laboratory Investigation·K Pietilä, T Koivula
Jul 1, 1985·Chest·I RubinsteinY Hiss
Jan 1, 1982·Clinical and Experimental Hypertension. Part A, Theory and Practice·T RosenthalA Yaron
Feb 16, 2006·American Journal of Physiology. Endocrinology and Metabolism·John R PepperellMaricruz Merino
Jan 1, 1991·Critical Reviews in Clinical Laboratory Sciences·B Bénéteau-Burnat, B Baudin
Mar 1, 1990·Hypertension·R DonnellyJ L Reid
Aug 13, 2017·Analytical and Bioanalytical Chemistry·Marija MladicJeroen Kool
Sep 1, 1984·Annals of Clinical Biochemistry·G A Maguire, C P Price

Citations

Nov 1, 1977·Analytical Biochemistry·A Persson, E B Wilson
Jun 24, 1976·The New England Journal of Medicine·J Lieberman, E Beutler
Jan 1, 1976·Annual Review of Biochemistry·R L Soffer
Feb 1, 1977·The Tohoku Journal of Experimental Medicine·M TakahashiK Yamauchi
Jul 1, 1971·Biochemical Pharmacology·D W Cushman, H S Cheung
Sep 18, 2008·Immunogenetics·Lynn M Herrmann-HoesingDonald P Knowles

Related Concepts

Cavia
Oligopeptides
Fluorescence Spectroscopy
Phosphoglycerate Dehydrogenase Activity
Fluorescent stain
Angiotensins
PHGDH wt Allele
2-aminohippuric acid
Assay
Serum

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