A fluorimetric assay of the phosphoribosylation of 6-mercaptopurine in human blood cells

Clinica Chimica Acta; International Journal of Clinical Chemistry
P C WongJ S Fowler


A new fluorimetric method to assay HGPRT (hypoxanthine-guanine phosphoribosyl transferase, EC from human red cell lysates using 6MP (6-mercaptopurine) as substrate is described. This assay is compared with an existing spectrophotometric method using hypoxanthine as substrate. Precision, sensitivity limits and kinetic differences of these assays are reported.


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Related Concepts

HPRT1 gene
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Hypoxanthine Phosphoribosyltransferase
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