A fluorimetric assay of the phosphoribosylation of 6-mercaptopurine in human blood cells

Clinica Chimica Acta; International Journal of Clinical Chemistry
P C WongJ S Fowler

Abstract

A new fluorimetric method to assay HGPRT (hypoxanthine-guanine phosphoribosyl transferase, EC 2.4.2.8) from human red cell lysates using 6MP (6-mercaptopurine) as substrate is described. This assay is compared with an existing spectrophotometric method using hypoxanthine as substrate. Precision, sensitivity limits and kinetic differences of these assays are reported.

Citations

May 1, 1977·Analytical Biochemistry·A Giacomello, C Salerno
Jun 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·W N KelleyJ E Seegmiller
Sep 1, 1973·Analytical Biochemistry·M A Schlossberg, V P Hollander
Dec 1, 1967·Analytical Biochemistry·J M Finkel
Oct 1, 1967·American Journal of Public Health and the Nation's Health·H C Chase
Apr 1, 1964·The American Journal of Medicine·M LESCH, W L NYHAN
Apr 4, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·D Andrew LoblawAmerican Society of Clinical Oncology

Related Concepts

Guanine
Mercaptopurine
HPRT1 gene
HPRT1 wt Allele
Fluorescence Spectroscopy
Hypoxanthine
Blood Cells
Hypoxanthine Phosphoribosyltransferase
Mercaptopurina Wellcome
Assay

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