PMID: 3767264Mar 1, 1986Paper

A fluorimetric method for measurement of erythrocyte transketolase activity

Annals of Clinical Biochemistry
S H Anderson, A D Nicol

Abstract

A method for measuring erythrocyte transketolase activity (ETKA) is described that is precise, economical of reagents and capable of high throughput with partial or full automation. The transketolase-dependent transformation of ribose-5-phosphate, yielding glyceraldehyde-3-phosphate, is linked via 'indicator enzymes' to glycerol production and NADH consumption, the latter being followed fluorimetrically. Conditions under which ETKA and 'TPP Effect' are derived have been examined and optimised. Values are comparable with those obtained by other methods but a relatively narrow adult reference range for ETKA is observed. Data are presented for optimal preparation and storage of samples.

References

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Citations

Jan 8, 2015·JPEN. Journal of Parenteral and Enteral Nutrition·Laura L Frank
Feb 1, 1997·Journal of the American College of Nutrition·J Eisinger
Nov 21, 1997·Annals of Clinical Biochemistry·C J Bates
Dec 24, 2020·Annals of the New York Academy of Sciences·Kerry S JonesAlbert Koulman

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