A fluorinated detergent for membrane-protein applications

Angewandte Chemie
Erik FrotscherSandro Keller

Abstract

Surfactants carrying fluorocarbon chains hold great promise as gentle alternatives to conventional hydrocarbon-based detergents for the solubilization and handling of integral membrane proteins. However, their inertness towards lipid bilayer membranes has limited the usefulness of fluorinated surfactants in situations where detergent-like activity is required. We demonstrate that fluorination does not necessarily preclude detergency, as exemplified by a fluorinated octyl maltoside derivative termed F6 OM. This nonionic compound readily interacts with and completely solubilizes phospholipid vesicles in a manner reminiscent of conventional detergents without, however, compromising membrane order at subsolubilizing concentrations. Owing to this mild and unusual mode of detergency, F6 OM outperforms a lipophobic fluorinated surfactant in chaperoning the functional refolding of an integral membrane enzyme by promoting bilayer insertion in the absence of micelles.

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Citations

Dec 24, 2015·The Journal of Organic Chemistry·Fréderic LegrandGrégory Durand
Dec 8, 2015·European Biophysics Journal : EBJ·Jonas M DörrJ Antoinette Killian
Oct 16, 2015·Biochimica Et Biophysica Acta·Watson LohKam Chiu Tam
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Jun 17, 2015·Analytical Biochemistry·Martin Textor, Sandro Keller
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Apr 11, 2019·Chemistry, an Asian Journal·Muhammad EhsanPil Seok Chae
Jul 7, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Mohamed DahaniFrançoise Bonneté
Feb 5, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Moheddine WehbieGrégory Durand
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Aug 16, 2019·The Journal of Organic Chemistry·Christophe BonnetGrégory Durand
Dec 12, 2020·Analytical Chemistry·Yuri M ShlyapnikovElena A Shlyapnikova

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