A fluorometric assay for α-glucosidase activity based on quaternary AgInZnS QDs.

Mikrochimica Acta
Jiabao ZhangXingguang Su

Abstract

A sensitive fluorescence strategy was constructed for the detection of α-glucosidase activity based on AgInZnS QDs. The AIZS QDs which were synthesized by hydrothermal method have a fluorescence emission wavelength of 554 nm. Ce4+ was able to oxidize p-phenylenediamine (PPD) to generate oxPPD, which can quench the fluorescence of AIZS QDs through dynamic quenching. When α-glucosidase was introduced into the system, L-ascorbic acid-2-O-α-D-glucopyranosyl (AAG) could be hydrolyzed to form ascorbic acid (AA), which can reduce Ce4+ and prevent the oxidation of PPD. Thus, the dynamic quenching process was blocked accompanying with the fluorescence recovery of AIZS QDs. The developed detection system for α-glucosidase displayed a good linear relationship between 0.01 and 0.16 U·mL-1 with a detection limit of 0.0073 U·mL-1. The sensing platform with high feasibility and anti-interference is a competitive alternative applied to α-glucosidase-related diagnostics.

References

May 29, 2008·Chemical Communications : Chem Comm·Dingsheng WangYadong Li
May 11, 2012·Advanced Materials·Tharangattu N NarayananPulickel M Ajayan
May 13, 2015·Luminescence : the Journal of Biological and Chemical Luminescence·Si-Yao LiuZhi-Qi Zhang
Jul 24, 2015·Biosensors & Bioelectronics·Juan ZhangGenxi Li
Jan 28, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yang ShengJunmin Xue

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