Nov 7, 2018

A folded conformation of MukBEF and Cohesin

BioRxiv : the Preprint Server for Biology
Frank BuermannJan Lowe


Structural maintenance of chromosomes (SMC)-kleisin complexes organize chromosomal DNAs in all domains of life, where they have key roles in chromosome segregation, DNA repair and regulation of gene expression. They function through topological entrapment and active translocation of DNA, but the underlying conformational changes are largely unclear. Using structural biology, mass spectrometry and cross-linking, we investigated the architecture of two evolutionarily distant SMC-kleisin complexes: proteobacterial MukBEF and eukaryotic cohesin. We show that both contain a dynamic coiled-coil discontinuity, the elbow, near the middle of their arms that permits a folded conformation. Bending at the elbow brings into proximity the hinge dimerization domain and the head/kleisin module, situated at opposite ends of the arms. Our findings favor SMC activity models that include a large conformational change in the arms, such as a relative movement between DNA contact sites during DNA loading and translocation.

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Mentioned in this Paper

C(2)M protein, Drosophila
Elbow Joint Structure
Planococcus citri gamma-proteobacterial endosymbiont
Decompression Sickness
DNA Repair
Regulation of Biological Process
Complex (molecular entity)
Memory Impairment

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