A food-grade fimbrial adhesin FaeG expression system in Lactococcus lactis and Lactobacillus casei

Canadian Journal of Microbiology
W W LuJian Kong

Abstract

Enterotoxigenic Escherichia coli (ETEC) infection is the major cause of diarrhea in neonatal piglets. The fimbriae as colonizing factor in the pathogenesis of ETEC constitute a primary target for vaccination against ETEC. Lactic acid bacteria (LAB) are attractive tools to deliver antigens at the mucosal level. With the safety of genetically modified LAB in mind, a food-grade secretion vector (pALRc or pALRb) was constructed with DNA entirely from LAB, including the replicon, promoter, signal peptide, and selection marker alanine racemase gene (alr). To evaluate the feasibility of the system, the nuclease gene (nuc) from Staphylococcus aureus was used as a reporter to be expressed in both Lactococcus lactis and Lactobacillus casei. Subsequently, the extracellular secretion of the fimbrial adhesin FaeG of ETEC was confirmed by Western blot analysis. These results showed that this food-grade expression system has potential as the delivery vehicle for the safe use of genetically modified LAB for the development of vaccines against ETEC infection.

References

Sep 1, 1978·Computer Programs in Biomedicine·M E Drets
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Apr 1, 2000·Applied and Environmental Microbiology·K I SørensenE Johansen
Aug 13, 2002·Applied Microbiology and Biotechnology·T M Takala, P E J Saris
Oct 31, 2002·Applied and Environmental Microbiology·Peter A BronPascal Hols
Nov 27, 2002·Applied and Environmental Microbiology·Isabelle BoucherSylvain Moineau
May 7, 2003·Current Opinion in Biotechnology·Michiel Kleerebezem, Jeroen Hugenholtz
Dec 22, 2004·Journal of Applied Microbiology·C-Q LiuN W Dunn
Apr 10, 2007·Microbial Cell Factories·Stéphane HazebrouckPhilippe Langella
May 27, 2009·International Journal of Medical Microbiology : IJMM·Katharina A RemerTobias A Oelschlaeger
Dec 25, 2010·Applied Microbiology and Biotechnology·Hassan MoeiniKhatijah Yusoff
Apr 21, 2011·Journal of Agricultural and Food Chemistry·Tien-Thanh NguyenClemens K Peterbauer
Jun 8, 2012·Molecular Biotechnology·Wenwei LuWentao Kong

❮ Previous
Next ❯

Citations

Dec 9, 2017·Frontiers in Microbiology·Wenyi ZhangZhihong Sun
Jan 19, 2019·Applied Microbiology and Biotechnology·Tina Vida Plavec, Aleš Berlec

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.