A formulation of pancreatic pro-enzymes provides potent anti-tumour efficacy: a pilot study focused on pancreatic and ovarian cancer

Scientific Reports
Macarena PeránJulian Kenyon

Abstract

Proteolytic enzymes have shown efficacy in cancer therapy. We present a combination of the two pro-enzymes Trypsinogen and Chymotrypsinogen A with potent in vitro and in vivo anti-tumour efficacy. A synergetic anti-tumour effect for Trypsinogen and Chymotrypsinogen A was determined at a ratio 1:6 (named PRP) using 24 human cancer cell lines. The antiangiogenic effect of PRP was analysed by matrigel-based tube formation and by fibrous capsule formation assays. Furthermore, cell invasion and wound healing assays together with qRT-PCR determination of epithelial-to-mesenchymal transition (EMT) markers were performed on human cancer cells treated with PRP. Additionally, in vivo pharmacokinetic studies were implemented and the PRP's anti-tumour efficacy was explored against orthotopic pancreatic and ovarian cancer tumours. PRP formulation was proven to inhibit in vitro angiogenesis, tumour growth, cancer cell migration and invasiveness; and to be an effective and well tolerated in vivo anti-tumour treatment. Finally, the clinical efficacy of a suppository formulation containing both pancreatic pro-enzymes in the context of a UK Pharmaceuticals Special Scheme was evaluated in advanced cancer patients. Consequently, PRP could have rel...Continue Reading

References

Aug 8, 1975·Science·C Leibow, S S Rothman
Apr 1, 1987·Biochemical Pharmacology·E ZivM Kidron
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Jan 5, 2002·Physiological Reviews·Stephen RothmanLois Isenman
Jul 18, 2002·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·X Z DingT E Adrian
Aug 22, 2002·Nature Reviews. Cancer·Jean Paul Thiery
Oct 29, 2002·Nature Cell Biology·William WoodPaul Martin
May 14, 2003·Stem Cells·Borhane AnnabiRichard Béliveau
Feb 18, 2004·Nature Reviews. Cancer·Ugo Cavallaro, Gerhard Christofori
Apr 21, 2004·Pancreas·Murat SarucParviz M Pour
Jun 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sanae NakajimaMasayuki Imamura
Feb 27, 2007·The American Journal of Pathology·James E TalmadgeAvraham Raz
Sep 4, 2008·Pancreas·Justin M M CatesCandice C Black
Jun 3, 2009·The Journal of Clinical Investigation·Raghu Kalluri, Robert A Weinberg
Jul 9, 2009·Cancer Research·Thiruvengadam ArumugamWoonyoung Choi
Nov 3, 2009·Cancer Letters·Daniele VergaraMichel Salzet
Mar 6, 2012·Cell·Wenjun GuoRobert A Weinberg
Sep 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Renhai CaoYihai Cao
Nov 10, 2013·Nature Medicine·Liling WanYibin Kang
Apr 25, 2014·Journal of Cellular and Molecular Medicine·Tze-Sing HuangMuh-Hwa Yang
May 23, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Sirin A I AdhamBrenda L Coomber
Dec 15, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Weixin LiangXiaoyu Gu

❮ Previous
Next ❯

Citations

Dec 17, 2019·Biomacromolecules·Anasua MukhopadhayayKamendra P Sharma
Apr 27, 2021·Expert Opinion on Biological Therapy·Aitor González-TitosMacarena Perán

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
Assay
confocal microscopy
scraping
PCR
column filtration

Software Mentioned

Elements
NIS
[UNK]
Excel

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.