PMID: 7539220May 1, 1995Paper

A forskolin-activated Cl- current in mouse mandibular duct cells

The American Journal of Physiology
A DinudomD I Cook

Abstract

We have previously shown that unstimulated granular duct cells of mouse mandibular gland contain a hyperpolarization-activated Cl- conductance with characteristics resembling the hyperpolarization-activated volume-sensitive Cl- channel (ClC-2). We now show that stimulation of these cells with forskolin, but not 1,9-dideoxyforskolin, activates a second whole cell Cl- conductance with properties resembling the cystic fibrosis transmembrane conductance regulator (CFTR). This conductance has a linear current-voltage relation and is not voltage activated. Its anion permeability sequence is Br- (1.96) > NO3- (1.36) > Cl- (1) > I- (0.44), and its conductance sequence is Cl- (1) > NO3- (0.66) > Br- (0.34) > I- (0.21). The current carried by this conductance is attenuated 65% by 1 mmol/l diphenylamine-2-carboxylate but is not affected by 0.1 mmol/l4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid or 0.1 mmol/l glibenclamide. The current can be activated by norepinephrine (1 mumol/l), evidently acting via beta-adrenergic receptors, since the effect of norepinephrine is inhibited by propranolol (1 mumol/l). We conclude that this adrenergically evoked conductance is due to CFTR, which has previously been shown to be expressed in salivary ...Continue Reading

Citations

Dec 23, 2009·The Journal of Physiology·Marcelo A CatalánJames E Melvin
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J E Melvin
Sep 20, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Victor G RomanenkoJames E Melvin

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