A four PDZ domain-containing splice variant form of GRIP1 is localized in GABAergic and glutamatergic synapses in the brain.

The Journal of Biological Chemistry
Erik I CharychAngel L De Blas

Abstract

We have isolated, from a rat brain cDNA library, a clone corresponding to a 2779-bp cDNA encoding a novel splice form of the glutamate receptor interacting protein-1 (GRIP1). We call this 696-amino acid splice form GRIP1c 4-7 to differentiate it from longer splice forms of GRIP1a/b containing seven PDZ domains. The four PDZ domains of GRIP1c 4-7 are identical to PDZ domains 4-7 of GRIP1a/b. GRIP1c 4-7 also contains 35 amino acids at the N terminus and 12 amino acids at the C terminus that are different from GRIP1a/b. In transfected HEK293 cells, a majority of GRIP1c 4-7 was associated with the plasma membrane. GRIP1c 4-7 interacted with GluR2/3 subunits of the alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid receptor. In low density hippocampal cultures, GRIP1c 4-7 clusters colocalized with GABAergic (where GABA is gamma-aminobutyric acid) and glutamatergic synapses, although a higher percentage of GRIP1c 4-7 clusters colocalized with gamma-aminobutyric acid, type A, receptor (GABA(A)R) clusters than with alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid receptor clusters. Transfection of hippocampal neurons with hemagglutinin-tagged GRIP1c 4-7 showed that it could target to the postsynaptic complex of GABAergi...Continue Reading

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Citations

Jun 5, 2012·PloS One·Csaba CserépGábor Nyiri
Aug 24, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yanfang LiAngel L De Blas
Jan 5, 2011·Neuropharmacology·Fernanda Laezza, Raymond Dingledine
Aug 14, 2009·The FEBS Journal·Jeannine MohrlüderDieter Willbold
Nov 7, 2006·Journal of Neurochemistry·Zi-Wei Chen, Richard W Olsen
Jul 22, 2006·The EMBO Journal·Laura E SwanStephan J Sigrist
Jan 18, 2008·Journal of Neurochemistry·Wendou Yu, Angel L De Blas
Sep 27, 2005·Molecular and Cellular Neurosciences·S B ChristieA L De Blas
May 11, 2011·Neuron·Bernhard LuscherCasey L Kilpatrick
Jul 19, 2016·Journal of Biomolecular Structure & Dynamics·Paulami Chatterjee, Debjani Roy
Nov 3, 2016·The Journal of Comparative Neurology·Christopher D FeketeAngel L De Blas
Sep 26, 2006·The Journal of Comparative Neurology·David R SerwanskiAngel L De Blas
May 25, 2005·The Journal of Comparative Neurology·Rong-Wen LiAngel L de Blas
Jul 1, 2010·The Journal of Comparative Neurology·Yanfang LiAngel L De Blas
Mar 17, 2006·Journal of Neurochemistry·Erik I CharychAngel L De Blas
Nov 24, 2006·The Journal of Biological Chemistry·Karina KulangaraHarald Hirling
Oct 15, 2019·The Journal of Comparative Neurology·Celia P MirallesAngel L De Blas
Sep 14, 2017·Developmental Neurobiology·Joshua M Lorenz-Guertin, Tija C Jacob
Mar 23, 2019·Frontiers in Cellular Neuroscience·Miranda MeleCarlos B Duarte
Nov 24, 2019·Journal of Cell Science·Alison E TwelvetreesJosef T Kittler
Apr 3, 2019·Cellular and Molecular Life Sciences : CMLS·Diane BissenAmparo Acker-Palmer
Jul 12, 2019·Frontiers in Molecular Neuroscience·Vladimir Khayenko, Hans Michael Maric
Feb 7, 2021·Cellular and Molecular Neurobiology·Shanu GeorgeAngel L De Blas

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