A Francisella tularensis DNA clone complements Escherichia coli defective for the production of Era, an essential Ras-like GTP-binding protein

Gene
M ZuberD L Court

Abstract

We cloned the era gene of Francisella tularensis from a plasmid library by heterologous genetic complementation of an Escherichia coli mutant conditionally defective for the production of Era, an essential protein for cell growth. Nucleotide sequence analysis indicated that, in F. tularensis, era constitutes a single gene operon. ORFs aspC and mdh encoding aspartate aminotransferase and malate dehydrogenase, respectively, flank era in F. tularensis. Although classified as Gram-, the flanking regions and the relative location of era in F. tularensis are distinctly different from those of typical Gram- and Gram+ bacteria. Computer analysis of bacterial Era protein sequences identified conserved domains in addition to the common G domains of most GTP-binding proteins.

References

Jan 1, 1991·Annual Review of Biochemistry·Y KaziroT Satoh
May 1, 1989·Reviews of Infectious Diseases·A Tärnvik

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Citations

Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·X ChenX Ji
Oct 3, 2007·Current Microbiology·Yong HuangSuMin Chen
Sep 3, 2011·Microbiology and Molecular Biology Reviews : MMBR·Natalie VerstraetenJan Michiels
Dec 8, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Eric D Brown
Aug 10, 1999·Journal of Bacteriology·B PowellD Court
Aug 28, 1999·Journal of Bacteriology·T I MeierG Zhao
Mar 28, 2002·Journal of Molecular Biology·Detlef D LeipeL Aravind

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