A FRET probe with AIEgen as the energy quencher: dual signal turn-on for self-validated caspase detection

Chemical Science
Youyong YuanBin Liu

Abstract

The accurate detection of biological substances is highly desirable to study various biological processes and evaluate disease progression. Herein, we report a self-validated fluorescent probe which is composed of a coumarin fluorophore as the energy donor and a fluorogen with aggregation-induced emission characteristics (AIEgen) as the energy quencher linked through a caspase-3 specific peptide substrate. Unlike the traditionally widely studied fluorescence resonance energy transfer (FRET) probes, our new generation of FRET probe is non-fluorescent itself due to the energy transfer as well as the dissipation of the acceptor energy through the free molecular motion of AIEgen. Upon interaction with caspase-3, the probe displays strong green and red fluorescent signals synchronously due to the separation of the donor-quencher and aggregation of the released AIEgen. The fluorescence turn-on with dual signal amplification allows real-time and self-validated enzyme detection with a high signal-to-background ratio, providing a good opportunity to accurately monitor various biological processes in a real-time manner.

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Citations

Dec 7, 2018·Chemical Society Reviews·Junji ZhangHe Tian
May 30, 2017·Chemical Science·Youyong Yuan, Bin Liu
Jul 23, 2020·Chemical Society Reviews·Luling WuTony D James
Mar 3, 2018·Chemical Society Reviews·Pengcheng ZhangHonggang Cui
Aug 25, 2018·Chemical Society Reviews·Hong-Wen LiuWeihong Tan
May 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiamin ChengBin Liu
Oct 8, 2020·Topics in Current Chemistry·Juliang YangFan Xia
May 15, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Julian BreidenbachMichael Gütschow
Jul 4, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Zhengwei HuangChuanbin Wu
Apr 8, 2021·Topics in Current Chemistry·Lei DongQing-Zheng Yang
Jul 18, 2019·Chemical Reviews·Duxia CaoWeiying Lin
Nov 16, 2017·ACS Applied Materials & Interfaces·Ju MeiHe Tian
Aug 23, 2019·Accounts of Chemical Research·Dong Wang, Ben Zhong Tang
Sep 6, 2017·Analytical Chemistry·Tae-Il KimYoungmi Kim
Jul 25, 2019·Accounts of Chemical Research·Minggang TianWeiying Lin
Dec 7, 2018·Chemical Reviews·Teresa L MakoMindy Levine
Oct 5, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Edward R H WalterNicholas J Long

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Methods Mentioned

BETA
FRET
biosensing
transmission electron microscopy

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