A fully automated amino acid analyzer using NBD-F as a fluorescent derivatization reagent

Biomedical Chromatography : BMC
Chiaki AoyamaKazuhiro Imai

Abstract

A fully automated amino acid analyzer using NBD-F (4- fluoro-7-nitro-2,1,3-benzoxadiazole) as a fluorescent derivatization reagent was developed. The whole analytical process was fully automated from derivatization, injection to HPLC separation and quantitation. The derivatization reaction conditions were re-evaluated and optimized. Amino acids were derivatized by NBD-F for 40 min at room temperature in the borate buffer (pH 9.5). The derivatives were separated within 100 min and fluorometrically detected at 540 nm with excitation at 470 nm. The detection limits for amino acids were in the range of 2.8-20 fmol. The calibration curves were linear over the range of 20 fmol to 20 pmol on column with the correlation coefficients of 0.999. The coefficients of variation were less than 5% at 3 pmol injection for all amino acids. Amino acids in rat plasma were determined by the proposed HPLC method.

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Citations

Apr 7, 2010·The Journal of Biological Chemistry·Janice A VrankaHans Peter Bächinger
Jan 14, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Chiaki AoyamaTakashi Funatsu
Mar 11, 2010·Journal of Clinical Biochemistry and Nutrition·Ying-Ling LaiHiroshi Ohshima
Jun 6, 2007·Biomedical Chromatography : BMC·T Cserháti
Nov 23, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Takeshi FukushimaToshimasa Toyo'oka
Mar 21, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Masayuki TomiyaToshimasa Toyo'oka
Aug 17, 2018·Journal of Experimental Botany·Matthew G GarneauMechthild Tegeder
Oct 12, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xiaomin LiMakoto Tsunoda
Feb 13, 2008·Journal of Separation Science·Hiroko UenoYoshinobu Baba
Dec 15, 2015·Nature Communications·Natsumi Ageta-IshiharaMakoto Kinoshita
Mar 20, 2020·Journal of Experimental Botany·Sandi Win ThuMechthild Tegeder
Oct 30, 2014·The Plant Journal : for Cell and Molecular Biology·Lizhi ZhangMechthild Tegeder
May 8, 2018·Analytical and Bioanalytical Chemistry·Rika SuzukiMakoto Tsunoda
Jan 3, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xue-Jiao ZhaoZhao-Ying Liu
Mar 17, 2021·Journal of Pharmaceutical and Biomedical Analysis·Takuya FujiwaraMakoto Tsunoda
Mar 27, 2016·Plant Physiology·James P Santiago, Mechthild Tegeder
Jan 22, 2019·Analytical Chemistry·Hisashi ShimizuTakehiko Kitamori

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