A fully automated effervescence-assisted switchable solvent-based liquid phase microextraction procedure: Liquid chromatographic determination of ofloxacin in human urine samples

Analytica Chimica Acta
Christina VakhAndrey Bulatov

Abstract

A novel fully automated effervescence-assisted switchable solvent-based liquid phase microextraction procedure has been suggested. In this extraction method, medium-chain saturated fatty acids were investigated as switchable hydrophilicity solvents. The conversion of fatty acid into hydrophilic form was carried out in the presence of sodium carbonate. The injection of sulfuric acid into the solution decreased the pH value of the solution, thus, microdroplets of the fatty acid were generated. Carbon dioxide bubbles were generated in-situ, and promoted the extraction process and final phase separation. The performance of the suggested approach was demonstrated by the determination of ofloxacin in human urine samples using high-performance liquid chromatography with fluorescence detection. This analytical task was used as a proof-of-concept example. Under the optimal conditions, the detector response of ofloxacin was linear in the concentration ranges of 3·10(-8)-3·10(-6) mol L(-1). The limit of detection, calculated from a blank test based on 3σ, was 1·10(-8) mol L(-1). The results demonstrated that the presented approach is highly cost-effective, simple, rapid and environmentally friendly.

References

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Mar 28, 2007·Analytica Chimica Acta·Mohammad Reza Khalili ZanjaniJan Ake Jönsson
Sep 25, 2007·Journal of Chromatography. a·Stig Pedersen-Bjergaard, Knut Einar Rasmussen
Jan 28, 2010·Analytica Chimica Acta·Shayessteh Dadfarnia, Ali Mohammad Haji Shabani
Mar 20, 2010·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hao WuLi-Ming Du
Jan 17, 2012·Analytica Chimica Acta·Wanessa R MelchertFábio R P Rocha
Sep 13, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Adriana M PimentaJosé L F Costa Lima
Dec 21, 2013·Analytica Chimica Acta·Guillermo Lasarte-AragonésMiguel Valcárcel
Apr 12, 2015·Journal of Pharmacological and Toxicological Methods·Christina VakhAndrey Bulatov
Sep 15, 2015·Critical Reviews in Analytical Chemistry·Christina VakhAndrey Bulatov

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Citations

Apr 25, 2019·Anais Da Academia Brasileira De Ciências·Mariana R GamaFábio R P Rocha
Feb 9, 2021·Journal of Chromatography. a·Eduardo CarasekJosias Merib
Jul 6, 2021·Ultrasonics Sonochemistry·Alena StuparIsabel Marrucho
Jan 7, 2020·Analytical Chemistry·Decibel P ElpaPawel L Urban
Sep 8, 2021·Journal of Separation Science·José GrauAmparo Salvador

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