A Fully Automated High-Throughput Flow Cytometry Screening System Enabling Phenotypic Drug Discovery

SLAS Discovery
John JoslinScott Lesley

Abstract

The goal of high-throughput screening is to enable screening of compound libraries in an automated manner to identify quality starting points for optimization. This often involves screening a large diversity of compounds in an assay that preserves a connection to the disease pathology. Phenotypic screening is a powerful tool for drug identification, in that assays can be run without prior understanding of the target and with primary cells that closely mimic the therapeutic setting. Advanced automation and high-content imaging have enabled many complex assays, but these are still relatively slow and low throughput. To address this limitation, we have developed an automated workflow that is dedicated to processing complex phenotypic assays for flow cytometry. The system can achieve a throughput of 50,000 wells per day, resulting in a fully automated platform that enables robust phenotypic drug discovery. Over the past 5 years, this screening system has been used for a variety of drug discovery programs, across many disease areas, with many molecules advancing quickly into preclinical development and into the clinic. This report will highlight a diversity of approaches that automated flow cytometry has enabled for phenotypic drug ...Continue Reading

References

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Citations

May 18, 2018·SLAS Discovery·Aaron C WilsonDouglas S Auld
Jun 18, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Kamila CzechowskaAttila Tárnok
Oct 22, 2019·European Journal of Immunology·Andrea CossarizzaArturo Zychlinsky
Jan 6, 2019·Biophysics Reviews·Ruth NussinovFeixiong Cheng

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Methods Mentioned

BETA
flow cytometry
FACS
leukapheresis
pull down
enzyme-linked immunosorbent assay
flow
ELISA

Software Mentioned

Segmenter
Dispatcher
FlowJo
Tibco
fcs
Spotfire

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