A fully integrated microchip system for automated forensic short tandem repeat analysis

The Analyst
Junping HanPeng Liu

Abstract

We have successfully developed an integrated microsystem that combines two plastic microchips for DNA extraction and PCR amplification with a glass capillary array electrophoresis chip together in a compact control and detection instrument for automated forensic short tandem repeat (STR) analysis. DNA extraction followed by an "in situ PCR" was conducted in a single reaction chamber of the microchip based on a filter paper-based extraction methodology. PCR products were then mixed with sizing standards by an injection electrode and injected into the electrophoresis chip for four-color confocal fluorescence detection. The entire STR analysis can be completed in about two hours without any human intervention. Since the 15-plex STR system has a more stringent requirement for PCR efficiency, we optimized the structure of the plastic DNA extraction and amplification chip, in which the reaction chamber was formed by sandwiching a hollow structure layer with two blank cover layers, to reduce the adsorption of PCR reagents to the surfaces. In addition, PCR additives, bovine serum albumin, poly(ethylene glycol), and more magnesium chloride were included into the on-chip multiplex STR system. The limit-of-detection study demonstrated tha...Continue Reading

References

Dec 12, 1985·Nature·P GillD J Werrett
Apr 26, 2003·Analytical Chemistry·Michael C BreadmoreJames P Landers
Oct 29, 2004·Nature Reviews. Genetics·Mark A Jobling, Peter Gill
Mar 29, 2006·Journal of Forensic Sciences·John M Butler
Dec 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Christopher J EasleyJames P Landers
Jun 8, 2007·Journal of Forensic Sciences·Katie M HorsmanJames P Landers
Dec 17, 2008·Forensic Science International. Genetics·Peng LiuRichard A Mathies
Dec 17, 2008·Forensic Science International. Genetics·Peter M ValloneJohn M Butler
Aug 28, 2009·Trends in Biotechnology·Peng Liu, Richard A Mathies
Oct 18, 2011·Journal of Chromatography. a·Michael C Breadmore
May 23, 2013·Journal of Forensic Sciences·Jenny A Lounsbury, James P Landers
Aug 7, 2013·Investigative Genetics·Eugene TanRichard F Selden
Aug 5, 2014·Forensic Science International. Genetics·Bobby L LaRueBruce Budowle
Sep 25, 2014·Lab on a Chip·Delphine Le RouxPhilippe de Mazancourt
Oct 7, 2014·Forensic Science International. Genetics·Mitchell Holland, Frank Wendt
May 13, 2015·Electrophoresis·Yong Tae KimTae Seok Seo
Mar 14, 2016·Forensic Science International. Genetics·Mavis Date-ChongMartin R Buoncristiani
Mar 22, 2016·Investigative Genetics·Rosemary S TuringanRichard F Selden

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.