A fully integrated, three-dimensional fluorescence to electron microscopy correlative workflow

Methods in Cell Biology
Claudia S LópezJoe W Gray

Abstract

While fluorescence microscopy provides tools for highly specific labeling and sensitive detection, its resolution limit and lack of general contrast has hindered studies of cellular structure and protein localization. Recent advances in correlative light and electron microscopy (CLEM), including the fully integrated CLEM workflow instrument, the FEI CorrSight with MAPS, have allowed for a more reliable, reproducible, and quicker approach to correlate three-dimensional time-lapse confocal fluorescence data, with three-dimensional focused ion beam-scanning electron microscopy data. Here we demonstrate the entire integrated CLEM workflow using fluorescently tagged MCF7 breast cancer cells.

Related Concepts

Related Papers

Methods in Cell Biology
Thomas Müller-Reichert, Paul Verkade
The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Katia CorteseCarlo Tacchetti
The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
J M RobinsonP R Cook
© 2022 Meta ULC. All rights reserved