PMID: 9420195Jan 7, 1998Paper

A functional antigenomic promoter for the paramyxovirus simian virus 5 requires proper spacing between an essential internal segment and the 3' terminus

Journal of Virology
S K MurphyG D Parks

Abstract

A previous analysis of naturally occurring defective interfering (DI) RNA genomes of the prototypic paramyxovirus simian virus 5 (SV5) indicated that 113 bases at the 3' terminus of the antigenome were sufficient to direct RNA encapsidation and replication. A nucleotide sequence alignment of the antigenomic 3'-terminal 113 bases of members of the Rubulavirus genus of the Paramyxoviridae family identified two regions of sequence identity: bases 1 to 19 at the 3' terminus (conserved region I [CRI]) and a more distal region consisting of antigenome bases 73 to 90 (CRII) that was contained within the 3' coding region of the L protein gene. To determine whether these regions of the antigenome were essential for SV5 RNA replication, a reverse genetics system was used to analyze the replication of copyback DI RNA analogs that contained a foreign gene (GL, encoding green fluorescence protein) flanked by 113 5'-terminal bases and various amounts of SV5 3'-terminal antigenomic sequences. Results from a deletion analysis showed that efficient encapsidation and replication of SV5-GL DI RNA analogs occurred when the 90 3'-terminal bases of the SV5 antigenomic RNA were retained, but replication was reduced approximately 5- to 14-fold in the ...Continue Reading

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Citations

Nov 21, 2001·The Journal of General Virology·V MiouletM D Baron
Dec 18, 2004·The Journal of General Virology·Diane VulliémozLaurent Roux
May 7, 2002·Journal of Virology·Philippe Le MercierDaniel Kolakofsky
Jun 17, 2005·The Journal of General Virology·Daniel KolakofskyRob W H Ruigrok
Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sarah L NotonRachel Fearns
Jun 2, 2015·Frontiers in Microbiology·Robert Cox, Richard K Plemper
Apr 14, 2016·The Journal of General Virology·Yusuke MatsumotoMachiko Nishio
Feb 16, 2006·The Journal of General Virology·Samuel Cordey, Laurent Roux
Nov 6, 2020·PLoS Pathogens·Irina GutscheDaniel Kolakofsky
Dec 16, 1998·Journal of Virology·R Fearns, P L Collins
Jun 9, 2006·The Journal of General Virology·Vanessa M CowtonRachel Fearns
Jan 24, 2018·RNA·Yusuke MatsumotoMachiko Nishio
Dec 28, 2018·RNA·Philippe le Mercier, Daniel Kolakofsky
Aug 19, 2007·The Journal of General Virology·Pramila Walpita, Clarence J Peters

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