A functional association between merlin and HEI10, a cell cycle regulator

Oncogene
Mikaela GrönholmOlli Carpén

Abstract

Merlin and ezrin are homologous proteins with opposite effects on neoplastic growth. Merlin is a tumor suppressor inactivated in the neurofibromatosis 2 disease, whereas upregulated ezrin expression is associated with increased malignancy. Merlin's tumor suppressor mechanism is not known, although participation in cell cycle regulation has been suggested. To characterize merlin's biological activities, we screened for molecules that would interact with merlin but not ezrin. We identified the cyclin B-binding protein and cell cycle regulator HEI10 as a novel merlin-binding partner. The interaction is mediated by the alpha-helical domain in merlin and the coiled-coil domain in HEI10 and requires conformational opening of merlin. The two proteins show partial subcellular colocalization, which depends on cell cycle stage and cell adhesion. Comparison of Schwann cells and schwannoma cultures demonstrated that the distribution of HEI10 depends on merlin expression. In transfected cells, a constitutively open merlin construct affected HEI10 protein integrity. These results link merlin to the cell cycle control machinery and may help to understand its tumor suppressor function.

References

Aug 14, 1998·Neurobiology of Disease·C RosenbaumC O Hanemann
Nov 24, 1999·The Journal of Biological Chemistry·C Gonzalez-AgostiV Ramesh
Feb 13, 2001·Trends in Cell Biology·P BurkhardS V Strelkov
Feb 27, 2001·Japanese Journal of Cancer Research : Gann·N MineM Emi
Nov 24, 2001·The Journal of Biological Chemistry·Guang-Hui XiaoJoseph R Testa
Jan 10, 2002·The Journal of Biological Chemistry·Joseph L KissilTyler Jacks
Aug 3, 2002·Nature Reviews. Molecular Cell Biology·Anthony BretscherRichard G Fehon
Jan 18, 2003·Experimental Cell Research·Megan Fabbro, Beric R Henderson
Jun 12, 2003·International Journal of Cancer. Journal International Du Cancer·Pascal PineauAnne Dejean
Aug 5, 2003·The Journal of Biological Chemistry·Mikaela GrönholmOlli Carpén
Dec 12, 2003·Nature Reviews. Cancer·Andrea I McClatchey
Jan 6, 2004·Nature Medicine·Chand KhannaLee J Helman
Mar 6, 2004·The Cancer Journal·Yumiko HirokawaHiroshi Maruta
Oct 19, 2004·Neuropathology and Applied Neurobiology·O TynninenA Paetau
Mar 9, 2005·Biochemistry·David N Chambers, Anthony Bretscher

❮ Previous
Next ❯

Citations

May 3, 2011·Acta Pharmacologica Sinica·Hong-zhi SunShu-fang Wu
Mar 4, 2008·Nature Reviews. Microbiology·Nicholas J BuchkovichJames C Alwine
Sep 22, 2012·Annual Review of Microbiology·Ping AnJames M Pipas
Feb 19, 2010·Cell Communication and Signaling : CCS·Chris J Moore, Steve J Winder
Sep 26, 2007·AJNR. American Journal of Neuroradiology·F J WippoldA Perry
Nov 6, 2007·Biochimica Et Biophysica Acta·Daniel R Scoles
Apr 20, 2007·Trends in Cell Biology·Tomoyo OkadaFilippo G Giancotti
Jul 24, 2015·Pflügers Archiv : European journal of physiology·B HanM Schmidt
Nov 22, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mei Lan TanTengku Sifzizul Tengku Muhammad
Jul 22, 2014·Oncogene·P A GuerreroD Marchetti
May 8, 2019·Bioscience, Biotechnology, and Biochemistry·Izzul IslamWataru Yoshida
Jul 13, 2019·The Journal of Biological Chemistry·Vinay MandatiDominique Lallemand
Apr 7, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Enrique CasadoPaloma Cejas
Aug 24, 2021·PloS One·Anne E RoehrigPablo Rodriguez-Viciana

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.