A functional chaperone triad on the yeast ribosome

Proceedings of the National Academy of Sciences of the United States of America
Matthias GautschiSabine Rospert

Abstract

The chaperones RAC (ribosome-associated complex), consisting of Ssz1p and zuotin, and Ssb1/2p are associated with ribosomes of yeast. Ssb1/2p was previously shown to form a crosslink product to polypeptides trapped in ribosome-nascent chain complexes (RNCs) in vitro. Here we show that an efficient crosslink of the nascent chain to Ssb1/2p depends on the presence of functional RAC. The crosslink to Ssb1/2p was significantly diminished if (i) RAC was removed from RNCs: a process reversed by addition of purified RAC; (ii) RAC carried a mutation in the J-domain of zuotin, leading to its inactivation in vivo; (iii) RAC's Ssz1p subunit was absent because RNCs were generated in a Deltassz1-derived translation extract. In vivo the same specific set of growth defects caused by the absence of any of the three chaperones was also displayed by a Deltassb1/2Deltassz1Deltazuo1 strain. The combination of in vitro and in vivo data supports a model in which Ssb1/2p, Ssz1p, and zuotin act in concert on nascent chains while they are being synthesized.

References

Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J HeitmanM N Hall
Jan 1, 1995·Methods in Enzymology·B S Glick, L A Pon
Jan 1, 1995·Methods in Enzymology·S Rospert, R Hallberg
Jul 12, 1996·Journal of Molecular Biology·Y Q QianD J McColl
Feb 26, 1998·Cell·B Bukau, A L Horwich
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·R GeorgeT Lithgow
Mar 6, 1998·Molecular and Cellular Biology·T C HallstromW S Moye-Rowley
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M K GreeneS J Landry
Aug 26, 1998·The EMBO Journal·W YanE A Craig
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·C S GässlerB Bukau
Oct 8, 1999·Molecular Biology of the Cell·U Fünfschilling, S Rospert
Apr 29, 2000·Cell·B BukauE A Craig
Aug 29, 2000·Science·T R Cech
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·M GautschiS Rospert
Apr 6, 2001·Cell·B A Maguire, R A Zimmermann
Jun 8, 2001·Annual Review of Biochemistry·J Frydman
Apr 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Heather HundleyElizabeth Craig

❮ Previous
Next ❯

Citations

Sep 3, 2008·Molecular Genetics and Genomics : MGG·Katarzyna Tomala, Ryszard Korona
Oct 3, 2003·Biochimica Et Biophysica Acta·Bostjan CernilaKatja Breskvar
May 7, 2003·Current Opinion in Microbiology·Elizabeth A CraigHeather A Hundley
Oct 2, 2004·Nature Reviews. Molecular Cell Biology·Jason C YoungF Ulrich Hartl
Jun 6, 2009·Nature Structural & Molecular Biology·Günter KramerBernd Bukau
Nov 13, 2012·Nature Structural & Molecular Biology·Basil J GreberNenad Ban
Dec 4, 2012·Nature Structural & Molecular Biology·Christoph LeidigIrmgard Sinning
Jun 4, 2005·Nature Structural & Molecular Biology·Bernd Bukau
Oct 6, 2006·Nature Structural & Molecular Biology·Peter C StirlingMichel R Leroux
May 24, 2005·Nature Structural & Molecular Biology·Peggy HuangElizabeth A Craig
Sep 25, 2010·The Journal of Biological Chemistry·Julia BenedixJohanna Dudek
Sep 3, 2009·Genes & Development·Ulrike von PlehweSabine Rospert
Apr 12, 2006·Eukaryotic Cell·Kristi L Muldoon-Jacobs, Jonathan D Dinman
Jan 20, 2011·Molecular and Cellular Biology·Himjyot JaiswalSabine Rospert
Oct 1, 2004·Molecular and Cellular Biology·Magdalena Rakwalska, Sabine Rospert
Apr 6, 2006·Genome Biology·Sabine Rospert, Agnieszka Chacinska
Apr 20, 2007·Cell Stress & Chaperones·Lance Shaner, Kevin A Morano
Nov 30, 2006·Biological Chemistry·Zdravko DragovicAndreas Bracher
Jan 3, 2012·Genetics·Kevin A MoranoW Scott Moye-Rowley
Nov 5, 2014·Nature Structural & Molecular Biology·Yixiao ZhangNing Gao
Jun 12, 2013·Annual Review of Biochemistry·Yujin E KimF Ulrich Hartl
Jul 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hendrik OttoSabine Rospert
Apr 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Heather HundleyElizabeth Craig
Jan 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Alison E MeyerElizabeth A Craig
Jun 2, 2005·Reviews of Physiology, Biochemistry and Pharmacology·S RospertY Dubaquié
Apr 7, 2010·The Journal of Cell Biology·Véronique AlbanèseJudith Frydman
Apr 7, 2010·The Journal of Cell Biology·Ansgar KoplinElke Deuerling
Oct 15, 2003·The Journal of Cell Biology·Masato YanoMasataka Mori
Jul 1, 2004·Molecular Microbiology·Helene C Eisenman, Elizabeth A Craig
May 22, 2007·Annals of the New York Academy of Sciences·Kevin A Morano
May 4, 2011·Biotechnology Journal·Sujata Jha, Anton A Komar
Oct 7, 2004·Biochemical and Biophysical Research Communications·Masayoshi YoshidaMasuo Obinata
Jun 5, 2004·Journal of Molecular Biology·Georgeta L DumitruJochen Reinstein
Oct 10, 2006·Biochemical and Biophysical Research Communications·Santanu RaychaudhuriAsim Dasgupta

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Matthias GautschiSabine Rospert
Proceedings of the National Academy of Sciences of the United States of America
Heather A HundleyE A Craig
Proceedings of the National Academy of Sciences of the United States of America
Hendrik OttoSabine Rospert
© 2022 Meta ULC. All rights reserved