A functional eukaryotic promoter is contained within the first intron of the hGH-N coding region

Biochemical and Biophysical Research Communications
A F KolbB Salmons

Abstract

Human growth hormone is frequently used as a reporter gene in studies addressing the regulation of eukaryotic promoters. Here we present evidence that the first intron of the hGH-N coding region contains a novel eukaryotic promoter which is able to direct the expression of hGH-N and luciferase reporter genes. The corresponding transcriptional initiation site is located downstream of the splice acceptor site of exon 2. This internal hGH-N promoter can interfere with the transcriptional control elements of a promoter linked to the hGH-N coding region and thereby complicate the interpretation of data obtained with hGH-N as a reporter gene.

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Citations

Apr 20, 2005·Letters in Applied Microbiology·L LiW Huang
Mar 22, 2005·The International Journal of Biochemistry & Cell Biology·Mingxu XuDalong Ma
Dec 16, 2003·Biochemical and Biophysical Research Communications·Mingxu XuDalong Ma
Jun 13, 2006·Developmental Biology·Jaebok Choi, Anna P Newman
Jun 25, 2003·Biochemical and Biophysical Research Communications·Andreas Kolb

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