PMID: 12781715Jun 5, 2003Paper

A functional interferon regulatory factor-1 (IRF-1)-binding site in the upstream regulatory region (URR) of human papillomavirus type 16

Virology
Istvan AranyStephen K Tyring

Abstract

We recently identified a new enhancer element (HIRE-1, HPV-Interferon Responsive Element-1) in the upstream regulatory region (URR) of human papillomavirus (HPV) type 16. HIRE-1 is located upstream from and in close proximity to the TATA box. HIRE-1 is 1 nt shorter in its 5' sequence in comparison to a consensus IRF-1 binding site (IRF-E). Gel shift analyses clearly demonstrated that HIRE-1 is capable of binding IRF-1 in response to interferon-gamma (IFN-gamma) treatment. In a reporter system, HIRE-1 stimulated transcription in response to IRF-1 or IFN gamma from both a heterologous or the homologous (p97) promoter in a dose-dependent manner. Mutations in the core binding sequence strongly decreased this enhancer activity. Interestingly, HIRE-1 stimulated transcription in the context of the full URR in a cell-type-specific manner, thereby suggesting the role of other cell-type-specific factors that might counteract with its function. Thus, our results may explain the inconsistent clinical and experimental results observed following IFN treatment of cervical lesions or cells. Also, this new enhancer may have an important function during inflammatory responses against HPV type 16.

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Citations

Sep 17, 2005·Kidney International·Istvan AranyRobert L Safirstein
Aug 30, 2008·Journal of Virology·Michael J LaceThomas H Haugen
Feb 12, 2013·Retrovirology·Mamneet Manghera, Renée N Douville
May 15, 2009·International Journal of Environmental Research and Public Health·Dorothy J WileyLisette M Johnson-Hill
Jul 19, 2005·Molecular Biology Reports·Meenakshi Upreti, Pramod C Rath
Jul 27, 2018·Molecular Neurobiology·Gerwyn MorrisBasant K Puri

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