A functional link between localized Oskar, dynamic microtubules, and endocytosis

Developmental Biology
Paulomi SanghaviGraydon B Gonsalvez

Abstract

Many cell types including developing oocytes, fibroblasts, epithelia and neurons use mRNA localization as a means to establish polarity. The Drosophila oocyte has served as a useful model in dissecting the mechanism of mRNA localization. The polarity of the oocyte is established by the specific localization of three critical mRNAs-oskar, bicoid and gurken. The localization of these mRNAs requires microtubule integrity, and the activity of microtubule motors. However, the precise organization of the oocyte microtubule cytoskeleton remains an open question. In order to examine the polarity of oocyte microtubules, we visualized the localization of canonical microtubule plus end binding proteins, EB1 and CLIP-190. Both proteins were enriched at the posterior of the oocyte, with additional foci detected within the oocyte cytoplasm and along the cortex. Surprisingly, however, we found that this asymmetric distribution of EB1 and CLIP-190 was not essential for oskar mRNA localization. However, Oskar protein was required for recruiting the plus end binding proteins to the oocyte posterior. Lastly, our results suggest that the enrichment of growing microtubules at the posterior pole functions to promote high levels of endocytosis in thi...Continue Reading

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Citations

Dec 9, 2014·RNA Biology·Timothy T Weil
Aug 16, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Atsushi Nakano, Seiji Takashima
Mar 28, 2016·Genetics·Paulomi SanghaviGraydon B Gonsalvez
Aug 12, 2016·Proceedings of the National Academy of Sciences of the United States of America·Wen LuVladimir I Gelfand

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