PMID: 8972387Dec 1, 1996Paper

A functional model for G protein activation of the muscarinic K+ channel in guinea pig atrial myocytes. Spectral analysis of the effect of GTP on single-channel kinetics

The Journal of General Physiology
Y HosoyaY Kurachi

Abstract

To elucidate the functional interaction between the active G protein subunit (GK*) and the cardiac muscarinic K+ (KACh) channel, the effect of intracellular GTP on the channel current fluctuation in the presence of 0.5 microM extracellular acetylcholine was examined in inside-out patches from guinea pig atrial myocytes using spectral analysis technique. The power density spectra of current fluctuations induced at various concentrations of GTP ([GTP]) were well fitted by the sum of two Lorentzian functions. Because the channel has one open state, the open-close transitions of the channel gate represented by the spectra could be described as C2<-->C1<-->O. As [GTP] was raised, the channel activity increased in a positive cooperative manner. The powers of the two Lorentzian components concomitantly increased, while the corner frequencies and the ratio of the powers at 0 Hz remained almost constant. This indicates that G protein activation did not affect the gating of each channel but mainly increased the number of functionally active channels in the patch to enhance the channel activity. Regulation of the number of functionally active channels could be described by a slow transition of the channel states, U (unavailable)<-->A (ava...Continue Reading

Citations

Mar 7, 1998·The Journal of General Physiology·A E AlekseevA Terzic
Aug 5, 1998·The Journal of General Physiology·T T Ivanova-NikolovaJ D Robishaw
May 19, 2010·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Shingo MurakamiYoshihisa Kurachi
Aug 19, 2003·The Journal of Physiology·Yoshihisa Kurachi, Masaru Ishii
Jan 23, 2016·Proceedings of the National Academy of Sciences of the United States of America·Nurit Degani-KatzavYoav Paas
Sep 14, 2013·Biochimica Et Biophysica Acta·Atsushi Inanobe, Yoshihisa Kurachi
Sep 21, 2013·Biophysical Journal·Shingo MurakamiYoshihisa Kurachi
Jan 21, 2010·Physiological Reviews·Hiroshi HibinoYoshihisa Kurachi
Jan 24, 2002·The Journal of Pharmacology and Experimental Therapeutics·Mitsuhiko Yamada
Dec 14, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·Zhi-Ren ZhangNael A McCarty
Oct 6, 2005·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Masaru Ishii, Yoshihisa Kurachi
Jan 16, 1998·Cellular Signalling·N Dascal

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