A functional model of hepatocyte transplantation for in vivo immunologic studies

Transplantation
Ginny L BumgardnerC G Orosz

Abstract

Previous studies to determine the significant donor and host factors which contribute to immune responses to allogeneic hepatocytes have been pursued with in vitro and in vivo studies. However, in order to further characterize in vivo host immune responses to transplanted hepatocytes in conjunction with their consequences upon allograft survival, a functional model of hepatocyte transplantation in which the function and survival of transplanted hepatocytes could be serially assessed was pursued. A transgenic mouse line expressing the human alpha1-antitrypsin (hA1AT) gene was developed in an FVB/N (H2q) mouse (hA1AT-FVB/N). Hepatocytes from these "donor" transgenic mice were transplanted into host mice by intrasplenic injection, and subsequent survival of the transgenic hepatocytes was determined by assay for the secreted hA1AT protein in host serum by enzyme-linked immunosorbent assay. Transplantation of transgenic "donor" hepatocytes (hA1AT-FVB/N) into (a) syngeneic FVB/N (H2q), (b) allogeneic immunoincompetent C57BL/6.SCID (H2b), or (c) allogeneic T cell-deficient (outbred) hosts resulted in long-term survival (> 16 weeks) of hepatocytes. Transplantation of allogeneic hepatocytes (hA1AT-FVB/N, H2q) into C57BL/6 (H2b), C3H (H2...Continue Reading

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Citations

Oct 2, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G L BumgardnerC G Orosz
Aug 11, 2001·Transplantation Proceedings·G Viberti
Sep 11, 1999·Journal of Gastroenterology and Hepatology·S M Riordan, R Williams
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Nov 21, 2000·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·N IchikawaN Murase
Jan 12, 1999·Transplantation·G L BumgardnerC G Orosz
Sep 28, 2004·Transplantation·D Denison JenkinsKarl G Sylvester
Oct 27, 2004·Transplantation·Keri E LunsfordGinny L Bumgardner
Aug 27, 2005·Transplantation·Phillip H HorneGinny L Bumgardner
Jan 13, 2009·Transplantation·Bing HanBo Qu
Dec 12, 2012·Transplantation·Jason M ZimmererGinny L Bumgardner
Dec 25, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kazuo OhashiMark A Kay
Aug 5, 2008·Journal of Pediatric Surgery·Scott V PerrymanKarl G Sylvester
Jun 16, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Donghong GaoGinny L Bumgardner
Sep 27, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Yue WangGinny L Bumgardner
Oct 24, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Cheng-Hsu ChenShiguang Qian
Jun 5, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K E LunsfordG L Bumgardner
Jan 26, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·I J FoxG R Yannam
Jan 29, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Konrad L StreetzMark A Kay
Aug 8, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K E LunsfordG L Bumgardner
Mar 5, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hong-Shiue ChouShiguang Qian
Jan 30, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J M ZimmererG L Bumgardner
Sep 16, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J M ZimmererG L Bumgardner
Dec 26, 2009·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Darjus F TschaharganehKonrad L Streetz
May 23, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Miwa MoritaShiguang Qian
Mar 3, 2012·European Journal of Pharmacology·Arindam PalArnold E Ruoho
Jul 6, 2016·Transplantation·Jason M ZimmererGinny L Bumgardner
Mar 25, 2016·World Journal of Transplantation·Felix OldhaferFlorian W R Vondran
Jul 17, 2012·Cell Transplantation·Soichi TakagiTeruo Okano
Nov 7, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jason M ZimmererGinny L Bumgardner
Dec 6, 2018·Current Opinion in Organ Transplantation·Travis D HullJoren C Madsen
Oct 13, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Horng-Ren YangShiguang Qian
Mar 5, 2019·Transplantation·Jason M ZimmererGinny L Bumgardner
Jan 13, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yan LiFeng Lin

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