A functional Small Ubiquitin-like Modifier (SUMO) interacting motif (SIM) in the gibberellin hormone receptor GID1 is conserved in cereal crops and disrupting this motif does not abolish hormone dependency of the DELLA-GID1 interaction

Plant Signaling & Behavior
Stuart NelisAri Sadanandom

Abstract

Plants survive adversity by modulating their growth in response to changing environmental signals. The phytohormone Gibberellic acid (GA) plays a central role in regulating these adaptive responses by stimulating the degradation of growth repressing DELLA proteins which accumulate during stress. The current model for GA signaling describes how this hormone binds to its receptor GID1 so promoting association of GID1 with DELLA, which then undergoes ubiquitin-mediated proteasomal degradation. Recent data revealed that conjugation of DELLAs to the Small Ubiquitin-like Modifier (SUMO) protein enables plants to modulate its abundance during environmental stress. This is achieved by SUMOylated DELLAs sequestering GID1 via its SUMO interacting motif (SIM) allowing non-SUMOylated DELLAs to accumulate leading to growth restraint under stress and potential yield loss. We demonstrate that GID1 proteins across the major cereal crops contain a functional SIM able to bind SUMO1. Site directed mutagenesis and yeast 2 hybrid experiments reveal that it is possible to disrupt the SIM-SUMO interaction motif without affecting the GA dependent DELLA-GID1 interaction and thereby uncoupling SUMO-mediated inhibition from DELLA degradation. Arabidopsis...Continue Reading

Citations

Mar 26, 2016·Journal of Experimental Botany·Gary YatesAri Sadanandom
Oct 21, 2016·Plant Reproduction·Alberto CampanaroLucio Conti
Dec 19, 2017·Frontiers in Plant Science·Magdalena J MazurHarrold A van den Burg
Dec 6, 2017·Journal of Integrative Plant Biology·Erbao ZhanHonghui Lin
Oct 18, 2019·Frontiers in Plant Science·Rebecca Morrell, Ari Sadanandom
Sep 5, 2020·Plant & Cell Physiology·Noel Blanco-TouriñánDavid Alabadí
Jul 29, 2021·Plant Science : an International Journal of Experimental Plant Biology·Danlu HanChengwei Yang

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Methods Mentioned

BETA
transgenic
salt stress
two hybrid
2 hybrid
ubiquitination

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