A functional transcriptomic analysis in the relict marsupial Dromiciops gliroides reveals adaptive regulation of protective functions during hibernation

Molecular Ecology
Roberto F NespoloFrancisco Bozinovic

Abstract

The small South American marsupial, Dromiciops gliroides, known as the missing link between the American and the Australian marsupials, is one of the few South American mammals known to hibernate. Expressing both daily torpor and seasonal hibernation, this species may provide crucial information about the mechanisms and the evolutionary origins of marsupial hibernation. Here, we compared torpid and active individuals, applying high-throughput sequencing technologies (RNA-seq) to profile gene expression in three D. gliroides tissues and determine whether hibernation induces tissue-specific differential gene expression. We found 566 transcripts that were significantly up-regulated during hibernation (369 in brain, 147 in liver and 50 in skeletal muscle) and 339 that were down-regulated (225 in brain, 79 in liver and 35 in muscle). The proteins encoded by these differentially expressed genes orchestrate multiple metabolic changes during hibernation, such as inhibition of angiogenesis, prevention of muscle disuse atrophy, fuel switch from carbohydrate to lipid metabolism, protection against reactive oxygen species and repair of damaged DNA. According to the global enrichment analysis, brain cells seem to differentially regulate a c...Continue Reading

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Citations

Oct 23, 2018·The FEBS Journal·Hanane Hadj-Moussa, Kenneth B Storey
Nov 16, 2019·BMC Genomics·Íria Gabriela Dias Dos SantosErika Cristina Jorge
Nov 25, 2020·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Jihui ZhangXiaobing Wu
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Nov 30, 2019·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Liam J Hawkins, Kenneth B Storey
Feb 9, 2021·Frontiers in Physiology·Sylvain GiroudKenneth B Storey
Sep 23, 2021·Scientific Reports·Sylvain GiroudStéphane Blanc

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