A functionalized, injectable hydrogel for localized drug delivery with tunable thermosensitivity: synthesis and characterization of physical and toxicological properties

Journal of Controlled Release : Official Journal of the Controlled Release Society
Paul Z EliasSuzie H Pun

Abstract

Thermosensitive injectable hydrogels have been used for the delivery of pharmacological and cellular therapies in a variety of soft tissue applications. A promising class of synthetic, injectable hydrogels based upon oligo(ethylene glycol) methacrylate (OEGMA) monomers has been previously reported, but these polymers lack reactive groups for covalent attachment of therapeutic molecules. In this work, thermosensitive, amine-reactive and amine-functionalized polymers were developed by incorporation of methacrylic acid N-hydroxysuccinimide ester or 2-aminoethyl methacrylate into OEGMA-based polymers. A model therapeutic peptide, bivalirudin, was conjugated to the amine-reactive hydrogel to investigate effects on the polymer thermosensitivity and gelation properties. The ability to tune the thermosensitivity of the polymer in order to compensate for peptide hydrophilicity and maintain gelation capability below physiological temperature was demonstrated. Cell encapsulation studies using an H9 T-cell line (CD4+) were conducted to evaluate feasibility of the hydrogel as a carrier for cellular therapies. Although this class of polymers is generally considered to be non-toxic, it was found that concentrations required for gelation were ...Continue Reading

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Citations

Jun 18, 2016·ACS Applied Materials & Interfaces·Hongchun WuDavid L Kaplan
Jul 18, 2016·Drug Discovery Today·Mohammad NorouziDonald W Miller
Oct 29, 2018·Advanced Healthcare Materials·Daniel C LeeSuzie H Pun
Jan 15, 2019·Biomaterials Science·Kewen Lei, Li Tang
Apr 8, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Peter AbdouZhen Gu
Nov 5, 2019·Frontiers in Chemistry·Dao-Yang FanZhong-Jun Liu
Feb 2, 2021·Colloids and Surfaces. B, Biointerfaces·Yin XiaoXiaoai He
Feb 5, 2021·International Journal of Pharmaceutics·Mohammad NorouziDonald W Miller
Aug 10, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Daniel C LeeSuzie H Pun
Mar 17, 2017·Accounts of Chemical Research·Nuria OlivaNatalie Artzi
Dec 9, 2019·ACS Biomaterials Science & Engineering·Caihong ZhuDavid L Kaplan

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