A further study on the combined use of internal standard and isotope-labeled derivatization reagent for expansion of linear dynamic ranges in liquid chromatography-electrospray mass spectrometry

Biomedical Chromatography : BMC
Yuhki TsukamotoTakashi Funatsu

Abstract

The combined use of a so-called internal standard and the isotope-labeled derivatization reagent for the quantification of analytes for liquid chromatography-mass spectrometry (LC/MS) was further studied. The sample solution (containing the analytes and an internal standard) was derivatized with the light form of the derivatization reagent, 7-(N,N-dimethylaminosulfonyl)-4-(aminoethyl)piperazino-2,1,3-benzoxadiazole (DBD-PZ-NH(2)) or 7-(N,N-dimethylaminosulfonyl)-4-piperazino-2,1,3-benzoxadiazole (DBD-PZ). A standard solution of the analytes (containing an internal standard) was derivatized with the isotope (d(6))-labeled derivatization reagent, DBD-PZ-NH(2) (D) or DBD-PZ (D), and served as the isotope-labeled internal standards. The peak heights of the targeted analytes derivatives in the sample solution were corrected using those of the internal standard and the heavy form derivatives of the standards, and the calibration curves were constructed. The curve bending of the calibration curves caused by the ion suppression at the ion source was suppressed and the linear dynamic ranges of the calibration curves were expanded. The derivatives of DBD-PZ-NH(2) were about 10 times more sensitively detected than those of DBD-PZ derivati...Continue Reading

References

Jan 1, 1989·Journal of Pharmaceutical and Biomedical Analysis·K ImaiT Toyo'oka
Aug 17, 2001·Biomedical Chromatography : BMC·S UchiyamaK Imai
Nov 28, 2001·Analytical Chemistry·R ZhangF E Regnier
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Nov 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Alexander Leitner, Wolfgang Lindner

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Citations

May 1, 2013·Journal of Chromatography. a·Per BruheimSilas G Villas-Boas
May 12, 2012·Journal of Pharmaceutical and Biomedical Analysis·Toshimasa Toyo'oka
Dec 7, 2007·Biomedical Chromatography : BMC·Tomofumi SantaKazuhiro Imai
May 22, 2007·Analytical Chemistry·R K Gilpin, C S Gilpin

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