A G alpha subunit gene is essential for conidiation and potassium efflux but dispensable for pathogenicity of Alternaria alternata on citrus.

Current Genetics
Nan-Yi WangKuang-Ren Chung

Abstract

Heterotrimeric G proteins play a profound role in the recognition and transduction of extracellular signals in eukaryotic cells. We characterized the AaG alpha1 gene, encoding a fungal Class I G alpha subunit of the GTP-binding protein, in Alternaria alternata of citrus. Interruption of AaG alpha1 with a marker gene resulted in fungal transformants producing fewer conidia, becoming hypersensitive to KCl, and displaying elevated lipolytic and pectolytic activities. Expression of a functional copy of AaG alpha1 in a null mutant restored all altered phenotypes to the wild type. The AaG alpha1 mutants, whose conidia germinate normally, caused necrotic lesions on citrus indistinguishable from wild type. Application of cAMP, its inhibitors (atropine and theophylline), or 3-isobutyl-1-methylxanthine (IBMX) decreased conidiation in the fungal strains carrying a functional AaG alpha1. In contrast, conidial formation in the null mutants was restored by dibutyryl-cAMP or by a low concentration of cAMP or theophylline (1 mM). Unlike the oxidative stress-responsive AaAP1 transcription activator or the AaFUS3 mitogen-activated protein kinase (MAPK), AaG alpha1 is not required for cellular resistance to oxidative, osmotic, or chemical stress....Continue Reading

References

Jan 1, 1978·Applied and Environmental Microbiology·D H HubbellM Umali-Garcia
Apr 27, 1977·Biochimica Et Biophysica Acta·D M OgrydziakS R Tannenbaum
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Boominathan, C A Reddy
Apr 15, 1997·The EMBO Journal·E RegenfelderR Kahmann
Dec 9, 1997·Molecular Plant-microbe Interactions : MPMI·S Liu, R A Dean
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·T H AdamsJ H Yu
Jan 26, 1999·Fungal Genetics and Biology : FG & B·M Bölker
Mar 11, 1999·Fungal Genetics and Biology : FG & B·B A HorwitzB G Turgeon
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·S Gao, D L Nuss
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·K B LengelerJ Heitman
Nov 1, 2001·Fungal Genetics and Biology : FG & B·J Wendland
Jan 5, 2002·Molecular Plant-microbe Interactions : MPMI·C S GronoverB Tudzynski
Jun 1, 2002·Science·Susana R NevesRavi Iyengar
May 31, 2003·Receptors & Channels·Peter Chidiac, Anju A Roy
May 15, 2004·Molecular Plant-microbe Interactions : MPMI·Peter S SolomonRichard P Oliver
Mar 2, 2005·Trends in Microbiology·Jesús AguirreWilhelm Hansberg
Aug 5, 2005·Annual Review of Phytopathology·Jae-Hyuk Yu, Nancy Keller
Nov 1, 2006·Molecular Plant-microbe Interactions : MPMI·Daisuke YamagishiMotoichiro Kodama
May 18, 2007·Annual Review of Microbiology·Liande LiKatherine A Borkovich
Sep 1, 2006·Medical Mycology·Jesús AguirreRosa Navarro

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Citations

Oct 12, 2012·World Journal of Microbiology & Biotechnology·Hsieh-Chin TsaiKuang-Ren Chung
Mar 27, 2013·Molecular Plant Pathology·Siwy Ling Yang, Kuang-Ren Chung
Jul 11, 2020·Indian Journal of Microbiology·Ruyi WangPingfang Tian

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