A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis

Nature
T YokomizoT Shimizu

Abstract

Leukotriene B4 (LTB4) is a potent chemoattractant that is primarily involved in inflammation, immune responses and host defence against infection. LTB4 activates inflammatory cells by binding to its cell-surface receptor (BLTR). LTB4 can also bind and activate the intranudear transcription factor PPAR alpha, resulting in the activation of genes that terminate inflammatory processes. Here we report the cloning of the complementary DNA encoding a cell-surface LTB4 receptor that is highly expressed in human leukocytes. Using a subtraction strategy, we isolated two cDNA clones (HL-1 and HL-5) from retinoic acid-differentiated HL-60 cells. These two clones contain identical open reading frames encoding a protein of 352 amino acids and predicted to contain seven membrane-spanning domains, but different 5'-untranslated regions. Membrane fractions of Cos-7 cells transfected with an expression construct containing the open reading frame of HL-5 showed specific LTB4 binding, with a K(d) (0.154nM) comparable to that observed in retinoic acid-differentiated HL-60 cells. In CHO cells stably expressing this receptor, LTB4 induced increases in intracellular calcium, D-myo-inositol-1,4,5-triphosphate (InsP3) accumulation, and inhibition of ade...Continue Reading

References

Nov 3, 1995·The Journal of Biological Chemistry·K ChangY Takuwa
Jul 1, 1994·The Journal of Experimental Medicine·S FioreC N Serhan
Jun 23, 1993·Annals of the New York Academy of Sciences·R DegiulioG Ricevuti
Jan 1, 1996·Journal of Leukocyte Biology·C J RaportP W Gray
Feb 2, 1996·The Journal of Biological Chemistry·T YokomizoT Shimizu
Aug 2, 1996·The Journal of Biological Chemistry·G K AkbarS P Kunapuli
Jan 1, 1996·General Pharmacology·J F KlinkerR Seifert
Nov 7, 1996·Nature·P R DevchandW Wahli
Oct 15, 1996·Genomics·C OwmanS J Lolait

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Citations

Jan 31, 2007·Inflammatory Bowel Diseases·Karsten H WeylandtDaniel C Baumgart
Aug 7, 1999·Clinical Reviews in Allergy & Immunology·J Z Haeggström
Aug 7, 1999·Clinical Reviews in Allergy & Immunology·S E Dahlén
Jan 15, 2004·American Journal of Respiratory Medicine : Drugs, Devices, and Other Interventions·Mitchell H Grayson, Phillip E Korenblat
Mar 27, 2013·Modern Rheumatology·Bahman YousefiAbbas Mirshafiey
Apr 24, 2007·Current Allergy and Asthma Reports·Mitchell H Grayson, Phillip E Korenblat
Apr 29, 1998·Trends in Pharmacological Sciences·B S Khakh, C Kennedy
Apr 13, 2000·Biochimica Et Biophysica Acta·S Spiegel, S Milstien
Jul 24, 2012·Analytical Chemistry·Donghyuk Kim, Christy L Haynes
Apr 30, 2011·Chemical Reviews·Motonao Nakamura, Takao Shimizu
Sep 23, 2011·Chemical Reviews·Jesper Z Haeggström, Colin D Funk
Mar 1, 2012·Acta Pharmacologica Sinica·Lei Sun, Richard D Ye
May 2, 2002·Nature Medicine·Takahide NagaseTakao Shimizu
Jul 26, 2005·Nature Reviews. Drug Discovery·Colin D Funk
Sep 3, 2004·Nature Reviews. Immunology·Andrew D Luster, Andrew M Tager
May 23, 2006·Nature Reviews. Neuroscience·R Douglas Fields, Geoffrey Burnstock
Dec 1, 1999·British Journal of Pharmacology·D CommuniJ M Boeynaems
Mar 1, 2000·British Journal of Pharmacology·G LenzJ T Neary
Feb 13, 2001·British Journal of Pharmacology·R Janssens, J M Boeynaems
Mar 27, 2002·The Pharmacogenomics Journal·E S SilvermanJ M Drazen
Aug 5, 1999·The Biochemical Journal·K MasudaT Shimizu
Jul 11, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M AiharaT Shimizu
Apr 17, 1999·Journal of Internal Medicine·H E Claesson, S E Dahlén
Jan 23, 1999·The New England Journal of Medicine·J M DrazenP M O'Byrne
Nov 8, 2002·The New England Journal of Medicine·Jonathan P Arm, K Frank Austen
Mar 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Richard EinsteinStephen B Liggett
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Simona ZariniAngelo Sala
Nov 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Antonio Di GennaroJesper Z Haeggström
May 29, 2009·The Journal of Biological Chemistry·Marita C BarthRobert E Gerszten
Oct 21, 2010·The Journal of Biological Chemistry·Fuyuki OkamotoTakehiko Yokomizo
Jul 1, 2011·The Journal of Biological Chemistry·Mariana Morato-MarquesCarlos H Serezani

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