PMID: 8580973Jan 1, 1996Paper

A galactose-rich, cell-wall glycoprotein from styles of Nicotiana alata

The Plant Journal : for Cell and Molecular Biology
J Sommer-KnudsenA Bacic

Abstract

A basic, galactose-rich style glycoprotein (GaRSGP) encoded by a previously characterized style-specific cDNA (NaPRP4) has been isolated from the styles of Nicotiana alata and structurally characterized. The glycoprotein is associated with cell walls in the transmitting tract and is composed of approximately 25% (w/w) protein and 75% (w/w) carbohydrate. The purified glycoprotein appears as a smear of between 45-120 kDa on SDS-PAGE; the deglycosylated protein backbone has an apparent molecular weight of approximately 30 kDa. The glycoprotein is rich in the amino acids lysine, proline, and hydroxyproline and in the monosaccharides galactose and arabinose. It is one of only a few proline/hydroxyproline-rich glycoproteins (P/HRGPs) to be characterized both as a cDNA-clone and protein. Glycans are attached to the protein backbone through both O- and N-glycosidic linkages with the majority of the carbohydrate being O-linked and consisting of short, highly branched chains terminating primarily in galactose residues. A carbohydrate epitope(s) is found on both GaRSGP and another style-specific glycoprotein but not on glycoproteins from other tissues. This finding provides further evidence for the existence of a style-specific carbohydra...Continue Reading

Citations

Nov 29, 2002·Journal of Experimental Botany·Felipe Cruz-GarciaBruce McClure
Apr 15, 2010·Plant Physiology·Miriam EllisAntony Bacic
Mar 11, 2004·The Plant Cell·Ana Maria SanchezCelestina Mariani
Dec 7, 2007·The Plant Cell·Yan-Hong ChenWei-Cai Yang
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Gladys I. Cassab
Jan 5, 2007·Annual Review of Plant Biology·Georg J Seifert, Keith Roberts
Mar 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jutta MessingAndreas Hensel
Aug 21, 2014·Annals of Botany·Derek T A LamportCharlotte E Seal
Mar 26, 2008·Plant Physiology and Biochemistry : PPB·Salvatore DeianaCaterina Senette
Apr 22, 2005·The Plant Journal : for Cell and Molecular Biology·Felipe Cruz-GarciaBruce McClure
Aug 24, 2005·The Plant Journal : for Cell and Molecular Biology·C Nathan HancockBruce A McClure
Oct 11, 2003·The Plant Journal : for Cell and Molecular Biology·Arjon J van Hengel, Keith Roberts
Jan 23, 2008·Proteomics·Elisabeth JametRafael Pont-Lezica
Dec 1, 2004·Annual Review of Genetics·Robert SwansonDaphne Preuss
Feb 7, 2015·Plant Reproduction·Ana Marta PereiraSílvia Coimbra
Jul 13, 2012·Annals of Botany·Eric Nguema-OnaAzeddine Driouich
May 18, 1999·The Journal of Biological Chemistry·J SvetekE A Nothnagel
Nov 8, 2005·Biochemistry. Biokhimii︠a︡·N I Rumyantseva
Jan 2, 2021·Frontiers in Plant Science·Jessy SilvaSílvia Coimbra
Sep 1, 2001·The New Phytologist·M J WheelerF C H Franklin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.