A gas chromatographic method for the determination of aldose and uronic Acid constituents of plant cell wall polysaccharides.

Plant Physiology
T M Jones, P Albersheim

Abstract

A major problem in determining the composition of plant cell wall polysaccharides has been the lack of a suitable method for accurately determining the amounts of galacturonic and glucuronic acids in such polymers. A gas chromatographic method for aldose analysis has been extended to include uronic acids. Cell wall polysaccharides are depolymerized by acid hydrolysis followed by treatment with a mixture of fungal polysaccharide-degrading enzymes. The aldoses and uronic acids released by this treatment are then reduced with NaBH(4) to alditols and aldonic acids, respectively. The aldonic acids are separated from the alditols with Dowex-1 (acetate form) ion exchange resin, which binds the aldonic acids. The alditols, which do not bind, are washed from the resin and then acetylated with acetic anhydride to form the alditol acetate derivatives. The aldonic acids are eluted from the resin with HCl. After the resin has been removed, the HCl solution of the aldonic acids is evaporated to dryness, converting the aldonic acids to aldonolactones. The aldonolactones are reduced with NaBH(4) to the corresponding alditols, dried and acetylated. The resulting alditol acetate mixtures produced from the aldoses and those from the uronic acids ...Continue Reading

References

Dec 1, 1967·Analytical Biochemistry·C W Nagel, S Hasegawa
Jan 1, 1970·Archives of Biochemistry and Biophysics·D F BatemanW W Kilgore
Dec 1, 1969·Analytical Biochemistry·D M Updegraff
Nov 1, 1967·Chemical & Pharmaceutical Bulletin·T Imanari, Z Tamura
Dec 1, 1967·Journal of Chromatography·Y S Ovodov, E V Evtushenko
Jul 10, 1967·Helvetica Chimica Acta·O RaunhardtH Neukom
Nov 1, 1964·Chemical & Pharmaceutical Bulletin·Z Tamura, T Imanari
Aug 1, 1966·Canadian Journal of Biochemistry·I M Morrison, M B Perry
Apr 28, 1961·Biochemical and Biophysical Research Communications·W J VANDENHEUVEL, E C HORNING
May 1, 1965·Canadian Journal of Biochemistry·M B PERRY, R K HULYALKAR
Jun 1, 1968·Plant Physiology·D J NevinsP Albersheim
Mar 1, 1969·Plant Physiology·F B Abeles

❮ Previous
Next ❯

Citations

Jul 15, 2005·Applied Biochemistry and Biotechnology·K W YuH J Suh
Mar 28, 1977·Biochimica Et Biophysica Acta·R P Allen, G J Mizejewski
Jan 3, 1978·Biochimica Et Biophysica Acta·G StrobelP Albersheim
Jul 2, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Masumi H SakuraiHaruki Yamada
Oct 20, 2005·Journal of Bioscience and Bioengineering·H MatsuyamaI Yumoto
May 1, 2013·Journal of Agricultural and Food Chemistry·Yuge NiuLiangli Lucy Yu
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M Wade, P Albersheim
Feb 1, 1988·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·S NotermansH W Engel
Jul 25, 2013·Journal of Asian Natural Products Research·Xiao-Qiang HanPeng-Fei Tu
Nov 1, 1972·Plant Physiology·W Loescher, D J Nevins
Jan 1, 1975·Plant Physiology·M McNeil, P Albersheim
Jan 1, 1979·Plant Physiology·K C Gross, S J Wallner
Feb 24, 2001·Biological & Pharmaceutical Bulletin·J I LeeH C Yang
Aug 21, 2001·Biological & Pharmaceutical Bulletin·H ChunH C Yang
Sep 17, 2002·Biological & Pharmaceutical Bulletin·Hyug ChunHan Chul Yang
Jul 3, 2002·Bioscience, Biotechnology, and Biochemistry·Byung-Keun YangChi-Hyun Song
Jul 5, 2003·Bioscience, Biotechnology, and Biochemistry·Byung-Keun YangChi-Hyun Song
Apr 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chang-Won ChoHee-Do Hong
Nov 1, 1977·European Journal of Biochemistry·R DatemaJ G Wessels

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.