A gated 31P NMR method for the estimation of phosphocreatine recovery time and contractile ATP cost in human muscle

NMR in Biomedicine
Jill M SladeRonald A Meyer

Abstract

Muscle phosphocreatine (PCr) recovery time constant (an index of muscle aerobic capacity) and contractile ATP cost were estimated from a gated (31)P NMR protocol which does not require intense, repetitive exercise. Subjects performed 2-s duration, maximum voluntary isometric ankle dorsiflexion contractions at 30-s intervals for 8 min (total 15 contractions), while single-shot (31)P spectra (51.7 MHz, TR 3 s) were acquired from the anterior compartment muscle. Spectra from the sixth through 15th contractions were retrospectively sorted, yielding 10 spectra (each 10 averages) gated to times before and after contraction. There was no significant decrease in muscle pH, allowing the calculation of contractile ATP cost directly from the percentage change in PCr during contraction cycles [8.86 +/- 0.82% (SE, n = 11) of PCr at rest], corresponding to an ATP cost of 1.69 +/- 0.16 mM/s (range 0.99-2.49 mM/s), assuming an 8.2 mM ATP concentration. The time constant for PCr recovery (tau 41.8 +/- 4.2 s, range 22.0-60.8 s) was calculated from tau = -Deltat/ln[D/(D + Q)], where Q is the percentage change in PCr due to contraction, D is the additional steady-state percentage drop in PCr from rest and Deltat is the interval between contraction...Continue Reading

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Citations

Jul 10, 2010·Current Opinion in Clinical Nutrition and Metabolic Care·Ian R Lanza, K Sreekumaran Nair
Oct 24, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sean C ForbesRonald A Meyer
Feb 19, 2011·Journal of Applied Physiology·Theodore F TowseRonald A Meyer
Jan 26, 2017·Analytical Biochemistry·Ladislav ValkovičMartin Krššák
Jul 15, 2017·Medicine and Science in Sports and Exercise·Anne TonsonJill M Slade
Oct 2, 2012·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Prodromos ParasoglouRavinder R Regatte
Feb 10, 2019·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Alejandro Santos-DíazMichael D Noseworthy
Feb 11, 2020·NMR in Biomedicine·Martin MeyerspeerUNKNOWN Experts' Working Group on 31P MR Spectroscopy of Skeletal Muscle
Jan 11, 2018·The International Journal of Medical Robotics + Computer Assisted Surgery : MRCAS·Hongbing LiKenji Kawashima

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