A Gbetagamma stimulated adenylyl cyclase is involved in Xenopus laevis oocyte maturation

Journal of Cellular Physiology
Leonardo GuzmánJuan Olate

Abstract

Xenopus laevis oocyte maturation is induced by the steroid hormone progesterone through a nongenomic mechanism that implicates the inhibition of the effector system adenylyl cyclase (AC). Recently, it has been shown that the G protein betagamma heterodimer is involved in oocyte maturation arrest. Since AC is the proposed target for Gbetagamma action, we considered of importance to identify and characterize the Gbetagamma regulated AC isoform(s) that are expressed in the Xenopus oocyte. Through biochemical studies, we found that stage VI plasma membrane oocyte AC activity showed attributes of an AC2 isoform. Furthermore, exogenous Gbetagamma was capable to activate oocyte AC only in the presence of the activated form of Galphas (Galphas-GTPgammaS), which is in agreement with the Ggammabeta conditional activation reported for the mammalian AC2 and AC4 isotypes. In order to study the functional role of AC in oocyte maturation we cloned from a Xenopus oocyte cDNA library a gene encoding an AC with high identity to AC7 (xAC7). Based on this sequence, we constructed a minigene encoding the AC-Gbetagamma interacting region (xAC7pep) to block, within the oocyte, this interaction. We found that microinjection of the xAC7pep potentiated ...Continue Reading

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Citations

Jun 19, 2007·BMC Pregnancy and Childbirth·Wei Yuan, Andrés López Bernal
May 25, 2006·Journal of Cellular Biochemistry·Silvana MartinezMaría Victoria Hinrichs
Oct 26, 2007·Journal of Cellular Physiology·Ximena RomoJuan Olate
Dec 23, 2006·Molecular Endocrinology·Liat Josefsberg Ben-YehoshuaJames L Maller
May 13, 2020·Cells·Catherine JessusEvelyn Houliston
Feb 4, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S L BlancoF San Juan

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