A GC-MS Protocol for Separating Endangered and Non-endangered Pterocarpus Wood Species

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Maomao ZhangYafang Yin

Abstract

Pterocarpus santalinus and Pterocarpus tincorius are commonly used traded timber species of the genus Pterocarpus. P. santalinus has been listed in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). As a non-CITES species, P. tincorius is also indiscriminately labeled as P. santalinus due to the similar macroscopic and microscopic features with P. santalinus. In order to understand the molecular discrimination between these easily confused species, xylarium heartwoods of these two species were extracted by three different kinds of solvents and analyzed using gas chromatography⁻mass spectrometry (GC-MS). Multivariate analyses were also applied for the selection of marker compounds that are distinctive between P. santalinus and P. tincorius. A total of twenty volatile compounds were detected and tentatively identified in three kinds of extracts, and these compounds included alcohols, stilbenoids, esters, aromatic hydrocarbons, ketones, miscellaneous, phenols, and flavonoids. GC-MS analyses also revealed that extraction solvents including ethanol and water (EW), ethyl acetate (EA), and benzene⁻ethanol (BE) gave the best chemotaxonomical discrimination in the chemical compone...Continue Reading

References

Aug 19, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiaoxia GaoWeimin Zhang
May 6, 2015·Nature Methods·Hiroshi TsugawaMasanori Arita
Jan 19, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ru LiuBin Lv
Aug 30, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaoqian YinFang Ma

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Methods Mentioned

BETA
PCA

Software Mentioned

MS
OPLS
SIMCA
SPSS
DIAL
DA

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