PMID: 9163908May 1, 1997Paper

A gene encoding a membrane protein exists upstream of the amoA/amoB genes in ammonia oxidizing bacteria: a third member of the amo operon?

FEMS Microbiology Letters
Martin G KlotzJeanette M Norton

Abstract

The gene cluster encoding ammonia monooxygenase (AMO) in the chemolithotrophic soil bacterium Nitrosospira sp. NpAV was found to contain a third open reading frame, termed amoC, upstream of the genes amoA and amoB that encode the subunits of AMO. The amoC gene and its flanking regions were isolated and sequenced from a 4.4 kb EcoRI fragment that contains one of three copies of the ammonia monooxygenase gene cluster. The presence of this gene upstream of the other two amoA gene copies in Nitrosospira NpAV as well as upstream of amoA genes in the genomes of other ammonia oxidizing nitrifiers (strains in the genera Nitrosomonas, Nitrosopira, Nitrosolobus and Nitrosovibrio) was confirmed using genomic DNA, oligodeoxyribonucleotide primers and the PCR. The amoC gene in Nitrosospira sp. NpAV encodes a 270 amino acid polypeptide of approximately 36 kDa. Topological analysis of the predicted primary structure revealed 6 membrane spanning domains. The amoC gene was expressed in recombinant Escherichia coli from its indigenous promoter.

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Citations

Mar 9, 2004·Journal of Microbiological Methods·Laia Calvó, L Jesús Garcia-Gil
Jul 17, 1998·Current Opinion in Chemical Biology·S J Ferguson
Aug 28, 2012·Applied and Environmental Microbiology·Roland Hatzenpichler
May 18, 2007·Annual Review of Microbiology·Daniel J ArpMartin G Klotz
Aug 24, 2006·Bioscience, Biotechnology, and Biochemistry·Ryuichi HirotaJunichi Kato
May 21, 2009·Biological Chemistry·Stefan GilchIngo Schmidt
Jul 31, 2012·Biochimica Et Biophysica Acta·Jörg Simon, Martin G Klotz
Sep 7, 2001·Annual Review of Microbiology·G A Kowalchuk, J R Stephen
Nov 7, 2016·Environmental Science and Pollution Research International·Pengcheng WangChengxiao Hu
Jun 6, 2018·Frontiers in Microbiology·Jackie K ZorzManuel Kleiner
Sep 5, 2014·Journal of the Air & Waste Management Association·Liang LiuPing Chen

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