Apr 1, 1994

A gene involved in control of human cellular senescence on human chromosome 1q

Molecular and Cellular Biology
P J HenslerOlivia M Pereira-Smith


Normal cells in culture exhibit limited division potential and have been used as a model for cellular senescence. In contrast, tumor-derived or carcinogen- or virus-transformed cells are capable of indefinite division. Fusion of normal human diploid fibroblasts with immortal human cells yielded hybrids having limited life spans, indicating that cellular senescence was dominant. Fusions of various immortal human cell lines with each other led to the identification of four complementation groups for indefinite division. The purpose of this study was to determine whether human chromosome 1 could complement the recessive immortal defect of human cell lines assigned to one of the four complementation groups. Using microcell fusion, we introduced a single normal human chromosome 1 into immortal human cell lines representing the complementation groups and determined that it caused loss of proliferative potential of an osteosarcoma-derived cell line (TE85), a cytomegalovirus-transformed lung fibroblast cell line (CMV-Mj-HEL-1), and a Ki-ras(+)-transformed derivative of TE85 (143B TK-), all of which were assigned to complementation group C. This chromosome 1 caused no change in proliferative potential of cell lines representing the othe...Continue Reading

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Mentioned in this Paper

Chromosomes, Human, Pair 1
Cell Aging
Specimen Type - Fibroblasts
Malignant Bone Neoplasm
Human Herpesvirus 5 species
Gene Deletion Abnormality
Chromosomes, Human
Thyroid Hormone Plasma Membrane Transport Defect

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