PMID: 6171594Jan 1, 1981Paper

A general approach to standardization of the solid-phase radioimmunoassay for quantitation of class-specific antibodies

Journal of Immunological Methods
W D Zollinger, J Boslego

Abstract

The feasibility of using an anti-human immunoglobulin/human immunoglobulin/[125I]anti-human immunoglobulin 'sandwich' in a solid-phase radioimmunoassay to produce a standard curve which could be used to quantitate antigen-specific antibody of a particular immunoglobulin class was investigated. The amount of secondary antibody (SAb) bound was determined as a function of whether the primary antibody (PAb) was bound to its specific solid-phase antigen or by a solid-phase anti-human immunoglobulin. No significant difference between the two values was observed. Quantitation of antitetanus toxoid antibody by this method was in good agreement with quantitative precipitin tests. Comparison of SAb binding as a function of the way the PAb is bound was extended to class-specific PAb by use of murine monoclonal antibodies to meningococcal antigens. In most cases somewhat greater binding of SAb occurred when PAb was bound to antigen, but in several cases where low avidity antibody and/or poor quality antigens were used, greater SAb binding occurred when PAb was bound by anti-mouse immunoglobulin. The results indicate that this approach may be useful as a general method for standardizing the SPRIA and other solid-phase immunoassays such as t...Continue Reading

Citations

Oct 29, 1982·Journal of Immunological Methods·O P Lehtonen, E Eerola
Feb 27, 1986·Journal of Immunological Methods·M W RussellJ Mestecky
Jun 26, 1987·Journal of Immunological Methods·J D BeattyL R Hill
Feb 9, 1990·Journal of Immunological Methods·B J WeiblenR Hoff
Apr 12, 1995·Journal of Immunological Methods·R K Gupta, G R Siber
Jun 1, 1988·The Journal of Allergy and Clinical Immunology·D Y LeungR S Geha
Jan 14, 1998·The Pediatric Infectious Disease Journal·J A EnglundG R Siber
Jul 3, 1999·The Pediatric Infectious Disease Journal·J B BrussG R Siber
Apr 1, 1990·Oral Microbiology and Immunology·J E ButlerS J Challacombe
Aug 20, 2011·Clinical and Vaccine Immunology : CVI·D GoldblattG M Carlone
Jul 16, 2002·Infection and Immunity·Jon B Bruss, George R Siber
Aug 1, 1986·The Journal of Clinical Investigation·D M AmbrosinoG R Siber
Jan 18, 2007·Molecular Medicine·Catherine H ValentineH Shaw Warren
Apr 1, 1986·Infection and Immunity·S J CryzR Germanier
Jan 1, 1991·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·R F Masseyeff
May 1, 1984·Clinical Immunology and Immunopathology·M Sandberg-WollheimT G Tachovsky
Oct 1, 1983·European Journal of Immunology·O Mäkelä, F Péterfy

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